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Vision Research Foundation

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+91-44-42271500


Larsen & Toubro Microbiology Research Centre and SAIL Molecular Microbiology Centre



Everyday, our eyes are constantly exposed to thousands of microbes that lodge on the outer surface of the eyes and the delicate linings that cover the eyeball. These microscopic organisms can invade the eye from the outside or enter into the eye from infection in the blood stream to cause sight-threatening diseases. Ocular microbiology is the study of microbial agents associated with infectious diseases of the eye and of the body’s response to these infections. Infectious organisms causing diseases include bacteria, fungi, viruses and parasites.

The research activities of the department are focused on studying the pathogenesis, rapid diagnosis and antimicrobial resistance in microbes that cause eye infections. The development of molecular diagnostic tests such as Polymerase Chain Reaction (PCR) and Gene sequencing for rapid and reliable detection of microbes associated with ocular infection are carried out in Molecular Microbiology section (SAIL Molecular Microbiology Research Centre) of the department.

The faculty include Dr. A.R. Anand (Senior Associate Professor), Dr. A.V.Kavitha (Assistant Professor), and Dr. M. Vimalin Jeyalatha (Research Associate). The department staff presently consists of 1 Senior executive, 5 Junior executives, 5 Laboratory Technicians, 1 junior research fellow, 1 project assistant, 2 Secretaries and2 Lab attenders. The routine activities of the department are carried out in 6 sections of Microbiology – Bacteriology, Mycology, Virology, Serology and Microbiological surveillance and Molecular microbiology.




Name & Email Designation
Dr A R Anand
dranand@snmail.org 		Senior Associate Professor
Mrs V Parameswari Senior Executive
Ms V Gayathri Junior Executive
Ms D Caroline Junior Executive
Ms B Saraswathi Junior Executive
Mr V Manoj Junior Executive
Ms S Jeevitha Junior Executive
Ms K Priyanga Laboratory Technician
Ms M Divya Laboratory Technician
Ms S Sumithra Laboratory Technician
Mr B Karthikeyan Laboratory Technician
Ms S Saranya Laboratory Technician
Ms Harinee Rajagopalan Project Assistant
Ms Thasni Fazil Junior Research Fellow
Ms A Arifa Begum Secretary
Ms S Sureka Secretary
Mr G Isreal Lab Attender



SIGNIFICANT CONTRIBUTIONS OF THE DEPARTMENT

This centre is the first in the country to standardize and apply molecular biological methods “In-house Polymerase Chain Reaction (PCR) techniques” for the detection of Eubacterial, Panfungal genomes from body fluids of patients with infectious etiology and specific genomes of Eubacterial genome, Panfungal genome, Propionibacterium acnes, Mycobacterium tuberculosis, Chlamydia trachomatis, Chlamydia pneumoniae , Herpes simplex virus , Varicella Zoster virus, Cytomegalovirus virus, Adenovirus, Rubella virus, Enterovirus and Coxsackie virus , Toxoplasma gondii and Acanthamoeba from ocular specimens.

These techniques have become the standard laboratory procedures to institute early specific therapy. Currently, the in-house PCR techniques standardized in this department are being utilized for rapid detection of infectious agents in clinical specimens by clinicians from 105 hospitals / clinics in and around Chennai through Vision Research Foundation Referral Laboratory ( Unit of Medical Research Foundation). The laboratory also caters to the ophthalmologists from five other states of India.

SAIL Molecular Microbiology Research Centre which is a part of L & T Microbiology Research Center was inaugurated by Shri P. Kulshrestha, Shri G.P. Shrivastava Regional Managers Southern region SAIL/Central Marketing Organisation Chennai on May 28th 2007.


Inauguration of SAIL Molecular Microbiology



LARSEN & TOUBRO MICROBIOLOGY RESEARCH CENTRE IS SHIFTED TO THE VI FLOOR OF NEW RESEARCH BUILDING (KNBIRVO BLOCK) FROM NOVEMBER 2009


MAJOR CONTRIBUTIONS


1. COMMERCIAL KIT FOR DETECTION OF ADENOVIRUS FROM OCULAR SPECIMENS BASED THE PUBLICATION FROM THIS CENTRE


Development and use of nested polymerase chain reaction (PCR) for the detection of adenovirus from conjunctivitis specimens. J. Clin. Virol 11: 77-84, 1998.

2. The first report of Isolation of Vesicular virus belonging to the family Rhabdoviridae from the aqueous humor of a patient with bilateral corneal endotheliitis

Ultrastructure features of Electron Microscopic picture of the isolated vesiculovirus from aqueous humor


Madhavan HN, Goldsmith CS, Rao SK, Fogla R, Malathi J and Priya K. Isolation of a vesicular virus belonging to the family rhabdoviridae from the aqueous humor of a patient with bilateral corneal endothelilitis, Cornea; 21 : 333-335, 2002.

Translational research contributions for laboratory diagnosis of ocular infections – In house PCR Techniques for rapid detection of aetiological agents


3. Indigenous PCRs for Rapid detection of infective agents in endophthalmitis

Detection of eubacterial / Propionibacterium acnes (P. acnes) / panfungal genome in intra-ocular specimens from clinically suspected endophthalmitis patients by application of in house nested Polymerase Chain Reaction (nPCR)

Polymerase chain reaction in the diagnosis of bacterial endophthalmitis. Br J Opthalmol. 82: 1078-1082, 1998.

Agarose gel electrophoretogram of amplified products of P.acnes from intraocular fluids



Agarose gel electrophoretogram of amplified products of Panfungal genome



Agarose gel electrophoretogram of Aspergillus PCR showing the amplified products of the four species of Aspergillus



Development of multiplex PCR for etiological diagnosis of infectious endophthalmitis



Detection of Non sporulating moulds (NSM) identified by Polymerase chain reaction (PCR) based DNA sequencing technique targeting internal transcribed spacer (ITS) region


Detection of Non sporulating moulds (NSM) identified by Polymerase chain reaction (PCR) based DNA sequencing technique targeting internal transcribed spacer (ITS) region



II. Infectious Etiology of Uveitis

A. Detection of Mycobacterium tuberculosis genome in intraocular fluids of granulomatous uveitis and other ocular specimens (1999 - 2004):
Mycobacterium tuberculosis DNA was detected in 18% (51 of 284) intraocular specimens including choroidal biopsies, subretinal mass aspirations, by application of in house nPCR using primers targeting MPB64 gene.

B. Association of mycobateria with Eales’ disease
Agarose gel electrophoretogram showing the amplified products of Mycobacterium tuberculosis



III. RAPID DETECTION OF VIRAL OCULAR INFECTIONS

Association of herpes viruses in the aqueous humor of patients with serpiginous choroiditis & Progressive Outer Retinal Necrosis (PORN)

PCR for CMV, HSV and VZV was carried out on nine patients with serpiginous choroiditis. Five were positive for VZV, one was positive for HSV suggesting the role of these viruses in pathogenesis. CMV DNA was detected by PCR in an individual with clinical diagnosis of PORN (HIV positive ) indicating the possible association of CMV in PORN.



Prevalence of HSV, VZV and CMV in HIV positive and HIV negative patients with of viral retinitis in India.

PCR for the detection of HSV, VZV and CMV was applied on intraocular fluids collected from 34 HIV positive and 99 HIV negative individuals. HSV infection was more prevalent among HIV negative patients, VZV was equally prevalent CMV infection was statistically more prevalent among the HIV positive patients.


Eur J Clin microbial Infect Dis 2004

Standardization and application of semi nested PCR for detection of the serotypes HSVI and HSV2 directly from ocular specimens.

Primers targeting the Glycoprotein D gene of HSV was standardised and applied onto a total of 18 intraocular fluids of Acute Retinal Necrosis (ARN) of which 4 were identified as HSV-2 and 3 as HSV-1.and in 7 cases of keratouveitis one was positive for HSV-2.


IJMM, 2005

Agarose gel electrophoretogram showing the amplified products of Herpes Simplex Virus serotypes 1 & 2



Genotyping of HSV – isolates:



Multiplex PCR for etiological diagnosis of ocular herpetic infections



IV. DETECTION OF HUMAN CYTOMEGALOVIRUS (HCMV) FROM CLINICAL SPECIMENS

1. Development of multiplex PCR for etiological diagnosis and quantitation of Human Cytomegalovirus infections

2. Standardization of pp65 antigenemia assay for semi-quantitation of late antigen of Cytomegalovirus in blood and Standardization of 5’ nuclease assay for Quantitation of viral load in clinical specimens positive for CMV.



3. Standardization of Multiplex PCR for genotyping of gB gene of Human Cytomegalovirus



4. Standardization of RFLP for identification of strain variations in gB, gH, gO, gL and gN regions of Cytomegaloviru



5. Analysis of mixed infections with multiple genotypes of Human Cytomegalovirus by RFLP and DNA sequencing



6. Analysis of mixed infections by multiple genotypes of Human Cytomegalovirus in Immunocompromised patients



ACHIEVEMENTS OF TRANSLATIONAL RESEARCH CLINICAL SPECIMENS PROCESSED BY APPLICATION OF INHOUSE POLYMERASE CHAIN REACTION (PCR) DURING JAN 2010 – DEC 2010 AT VRF REFERRAL LABORATORY


COMPLETED RESEARCH PROJECTS IN COLLOBORATON WITH INTERNATIONAL LABORATORIES

I. Alcon Laboratories Inc., USA: Nucleic acid based methods (including ribotyping) for direct species level detection of bacterial genome(s) and ribotyping of bacterial isolates from eyelid /lash swabs of Blepharitis patients.

Clinical trials and microbiological studies

  • A Clinical evaluation of efficacy and safety of 3% Ciprofloxacin Ophthalmic Solution in treating bacterial corneal ulcer.

  • A clinical and microbiological evaluation of efficacy and safety of 3% Ciprofloxacin Ophthalmic ointment relating bacterial corneal ulcers.

  • A study on bacterial corneal ulcers treated with “standard antibacterial therapy”

  • A double masked pilot study comparing Ciprofloxacin and Tetracycline ophthalmic ointments for treating conjunctivitis caused by Chlamydia trachomatis.

  • Double masked comparison of Ciloxan to Fortified Tobramycin/Cefazolin for treating bacterial Corneal Ulcers.

  • Efficacy and Safety of 1.0% Rimexolone Ophthalmic suspension versus Pred Forte for treatment of uveitis

  • An evaluation of the Safety and Efficacy of Moxifloxacin Ophthalmic Solution 0.5% Versus OCUFLOX Ophthalmic Solution 0.3% in the Treatment of Bacterial Conjunctivitis in India.

  • Microbiology of Healthy Eyes in India

  • Comparison of Ciprofloxacin 0.3% Dexamethasone 0.1% suspension , versus Ciprofloxacin ).3% Solution for treatment of Chronic Blepharitis

II. Waseda University, Tokyo, Japan:

In-vitro studies on the applications of Mebiol gel in cultivation of cells from eye tissues and growth of viruses in these cells and their susceptibility to antiviral drugs


IJMR,2006
Current science, 2004

Ex vivo cultivation of corneal Limbal epithelial cells in a Thermo-reversible polymer (Mebiol Gel) and their transplantation in Rabbits – an animal model.



COMPLETED RESEARCH PROJECTS IN COLLOBORATON WITH NATIONAL LABORATORIES

Cadila Pharmaceuticals Ltd, India:

Synergic activity of SN-198 and Amphotericin B SN-198 and Ketaconazole



“Synergistic activity of SN-198 and / or its modified product with the Amphotericin B and Fluconazole using the fungal strains which are resistant to Amphotericin B and SN-198 in our earlier study”


INTRAMURAL COMPLETED FUNDED PROJECTS
  • Effect of Mitomycin C on Fibroblast cell cultures grown from sub-conjunctival tissue from glaucoma patients. (Ophthalmic Surgery, 1995)

  • To study the aetological role of tubercle bacillus in Eales' disease from the serum, vitreous paraffin section of epiretinal, vitreous and subretinal membrane. (BJO 1999, IOVS 2000, IJO 2002)

  • Ampicillin and Sulbactum Assay in Aqueous Humor and Serum. (IJO, 1998)

  • Comparison of In-vitro activities of Tetracycline and Ciprofloxacin against C. trachomatis isolates for conjunctivitis. (IJMR, 1996)

  • Long term in-vitro effects of Mitomycin C treated human Tenon's capsule fibroblasts and their correlation with surgical outcome in trabeculectomy in Glaucoma patients. (IJMR, 1997)

  • Evaluation of direct diagnosis with culture confirmation using modified shell vial method for diagnosis of Chlamydia, Adenovirus and Herpes Simplex Virus (HSV) infection of cornea and conjunctiva in patients attending Sankara Nethralaya. (Afro-Asian J Ophthalmol, 1992)

  • Use of Polymerase chain reaction (PCR) and DNA probe Hybridization to Determine the Gram reaction of the infecting bacterium in the intraocular fluids of patients with Endophthalmitis (J Infection; 41: 221 -226)

  • Polymerase Chain Reaction to find the association of Chlamydophila (Chlamydia pneumoniae) in optic neuritis and papilledema - A pilot study (IJPM, 2007)

  • Standardisation Of Western Blotting Technique For Detection Of Anti-IgG and IgM against Reactive Antigens of Toxoplasma Gondii Tachyzoites.

  • Pilot Study - Etiology of Fuchs’ heterochromic uveitis A pilot study has been undertaken to evaluate the role of rubella, herpes zoster, herpes simplex and Toxoplasma gondii in the pathogenesis of Fuchs’ heterochromic uveitis and PCR is positive for Toxoplasma gondii genome in 3 and HSV genome in one of the 40 AH from patients with clinically suspected Fuchs’ heterochromic uveitis.

  • Study on the antiviral property of isatin derivatives and acyclovir prodrugs against isolates of Human Herpes Simplex Virus”.

COMPLETED PROJECTS THROUGH RESEARCH GRANTS EXTERNAL FUNDING AGENCIES

I. INDIAN COUNCIL OF MEDICAL RESEARCH (ICMR)

1. Title of the project : “Polymerase chain reaction for the detection and genotyping of Chlamydia trachomatis in conjunctivitis” (5/3/3/17/98 – ECD – I) (1999 – 2001)



Primers targeting plasmid and major outer membrane protein (MOMP) gene of Chlamydia trachomatis (C. trachomatis) were evaluated for their sensitivity and specificity in detecting the organism among conjunctival specimens. Plasmid primers were more sensitive in detecting C. trachomatis in 11(13.8%) whereas MOMP primers were able to amplify C. trachomatis DNA in only 3 among the 11 positive specimens

A total of 486 conjunctival specimens collected from individuals suspected to have inclusion conjunctivitis were processed for detecting of C. trachomatis. The overall rate of C. trachomatis positivity was 5.6 % . Isolation techniques were not successful. The predominate genotype was determined as Ba.


2. Molecular biological and virological study of intraocular fluids from patients with viral retinitis and choroiditis - correlation with clinical features and visual outcome. ( 5/4/6/1/2000 – NCD – II) (2001 – 2003)

PCR is a rapid diagnostic tool for aetiological diagnosis of viral retinitis. VZV was the most commonest cause of viral retinitis based on PCR and was detected in 28, CMV in 20 and HSV in 14 of 100 viral retinitis patients.

VZV DNA was detected in 5, and HSV in one among 9 patients with serpiginous choroiditis, pathogenesis of this disease.

PCR based RFLP is a reliable, less laborious and cost effective molecular biological tool for the determination of HSV serotypes both for the clinical isolates and culture negative PCR positive specimens.



3. Title of the project : Detection and molecular characterization of mycobacteria by Polymerase Chain Reaction (PCR) Restriction Fragment Length Polymorphism (RFLP) and DNA sequencing technique from ocular and other clinical specimens. ( 5/8/5/11/2000 – ECD - I ) (2000 – 2002)


4. Title of the project: “Prevalence of Rubella virus associated congenital cataract: detection of the virus in the lens aspirates ( 5/4/6/2/03 - NCD- II) (2005 – 2008)

Significant contributions: Congenital Rubella syndrome (CRS) occurs only when the mother acquires a primary Rubella infection during the first trimester. 20% of the children born after such an infection suffer the severe congenital abnormalities associated with CRS.



Reverse transcriptase Polymerase chain reaction (RT-PCR) was applied on 100 lens aspirates and peripheral blood and 23 (23%) were positive by nRT-PCR).

Among the 23 lens aspirates positive by nRT-PCR 13 were isolated on SIRC cell line.

Sequencing of the 13 culture isolates reveals two genotypes, Genotype I and Genotype II. 11 of these isolates belong to Genotype I and two belong to Genotype II. The genotyping was also confirmed by PCR –RFLP technique using Taq I enzyme and found to be in concordance with the results of the DNA sequencing.


5. Polymerase Chain Reaction for detection of Mycobacterium genus specific genome, Mycobacterium fortuitum and Mycobacterium chelonae genomes in vitreous and epiretinal membranes and blood of Eales’ disease patients.( 5/3/3/3/2004 – ECD - I) (2005 – 2008)

The aetiopathogenesis of Eales’ disease is not clearly understood because of lack of appropriate specimens for microbiologic study.

In this prospective study, 4 out of 45 (8.9%) vitreous aspirates from Eales’ disease patients were positive by PCR for Mycobacterium fortuitum (M. fortuitum), one for Mycobacterium chelonae (M. chelonae) by PCR and 3 for M. tuberculosis by PCR.

8 intraocular clinical specimens from Eales patient were positive for mycobacterium either / or by PCR and culture showed a statistically significant (P < 0.001) association of Mycobacetrium species with Eales’ disease.

Agarose gel electrophoretogram showing amplification of M. chelonae DNA targeting ITS gene from Eales’ patients Vitreous DNA



This is the first report mentioning the isolation of rapid growing nontuberculous mycobacteria (RGNTM) from the Vitreous aspirate of Eales’ patients with probable confirmation of their aetiopathogenic role in the disease process.


6. Application of Polymerase chain reaction (PCR) for the detection of Toxoplasma gondii in intraocular fluids from suspected cases of chorioretinits ( 5/3/3/3/2003- ECD - I ) (2005 – 2008)

Of the five different nPCRs standardized in this study- 2 targeting the B1 gene, one SAG1 and 2 for SAG2 (3’ end and 5’ end), T. gondii DNA was detected by at least one of the nPCR in intraocular fluids/peripheral blood leucocytes from 39.7% of 131 TRC patients including 74.1% of 27 HIV positive patients

The clinical diagnosis of ocular toxoplasmosis was supported by laboratory tests-WDC and nPCR in 68.8% of 77 TRC patients including 85.1% of 27 HIV positive TRC patients.

In 52 congenital cataract patients included in this study, T. gondii DNA was detected by at least one of the 4 nPCRs (2 targeting B1 gene and 2 targeting SAG2 gene) in 59.6% of lens aspirate and/or peripheral blood leucocytes. This is the first report in literature on the detection of T. gondii DNA by nPCR in lens aspirate of congenital cataract patients.



The association of T. gondii with Fuchs Heterochromic Iridocyclitis (FHI) was detected for the first time in India in this study by the presence of T. gondii DNA by nPCR targeting B1 gene in 18.2% of 33 AH. Genotyping of T. gondii by nPCR-RFLP and DNA sequencing: Genotype I in 41 patients consisting of 13 TRC patients ( including 7 HIV positive), 23 congenital cataract patients, and 5 FHI patients. This is the first study of genotyping of T. gondii from India.


7. Comparison of taqman probe based Real-Time polymerase chain reaction (RT-PCR) and pp65 antigenemia assay for the quantitation of human cytomegalovirus (HCMV) in clinical specimens and a study on ganciclovir resistance of HCMV (Order No 5/8/7/17/2006-ECD-I)

The objectives of the study were optimization of Taqman probe based real time PCR for detection and quantification of HCMV genome along with the application of pp65 antigenemia assay on to the clinical specimens obtained from immunocompromised patients ie. HIV patients, post renal transplant recipients with HCMV manifestations including HCMV retinitis. The results of pp65 antigenemia assay and Real-time PCR by applying the techniques prospectively on peripheral blood specimens obtained from these immunocompromised patients were correlated. Finally the novel ganciclovir resistance mutation by sequencing the UL-97 gene and UL-54 gene in the patients who do not respond to the treatment with ganciclovir or valgancyclovir as decided by the nephrologists or ophthalmologists were determined.

Qualitative nested PCR:

A total of 82 Peripheral blood specimens were collected from post renal transplant recipients and 82 healthy kidney donors, 20 HIV infected individuals with HCMV retinitis. DNA from the clinical specimens was extracted using Bioneer genomic extraction kits and stored at -20oc till further use. Qualitative nested PCR targeting the mtr II region of HCMV has been applied onto the DNA extracted from these specimens and the amplified product was detected by electrophoresis on 2% agarose gel incorporated with ethidium bromide. CMV AD-169 (ATCC-VR 538) serially passaged in WI-38 diploid cell line was used as positive control.


Observation & Results:

Fifty nine (71.95 %) of 82 post renal transplant patients, 11 (13.41 %) of 82 kidney donors and all 20 (100%) of 20 HIV patients with HCMV retinitis (either by blood DNA or by Aqueous humour DNA or by both) were positive for HCMV DNA, the copy number ranging from 1 copy/ml. to 8462847 copies/ml. The clinicians were prompted with the HCMV results of the donor for the further management and decision. In case of pp65 antigenemia assay thirty eight (46.34 %) of 82 post renal transplant patients, 1 (1.22 %) of 82 kidney donors and 13 (65 %) of 20 HIV patients with HCMV retinitis were positive by pp65 antigenemia assay. The clinicians were prompted with the HCMV results of the donor for further management and decision.

Comparative analysis of results of pp65 antigenemia assay with real time PCR


Total No. of specimens Real Time PCR pp65
Post transplant recipients 82 59 (71.95 %) 38 (46.34 %)
Kidney Donors 82 11 (13.41 %) 1 (1.22 %)
HIV patients with HCMV retinitis 20 20 (100%)* 13 (65 %)
Blood donors 20 0 (0 %) 0 (0 %)
Total 204 90 (44.11 %) 52 (25.49 %)+

Among the 204 specimens processed 52 were positive for both pp65 antigenemia and real time PCR and 38 more were positive only by real-time PCR.
The specimens were positive for HCMV DNA either for blood DNA or Aqueous humour DNA.s

Agarose Gel Electrophoretogram of PCR of Region I- Round II on Clinical Specimens




II. COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH (CSIR)

CSIR- NMITLI PROJECT

The project was aimed to develop a Xcyto-screen DNA chip as a diagnostic product of collaborative research of Ophthalmic centres viz. Vision Research Foundation, Chennai; L.V. Prasad Eye institute, Hyderabad; Dr. Rajendra Prasad Centre for Ophthalmic Sciences, AIIMS, New Delhi; Centre for Cell and Molecular biology, Hyderabad and industrial partner, Xcyton Diagnostics Pvt., Ltd., Bangalore. Multiplex Polymerase chain reaction based hybridization technique for simultaneous detection of infectious etiology of Keratoconjunctivitis (external ocular infections), viral Retinitis, Uveitis, infectious Endophthalmitis (intraocular infections) was standardized. The infective agents includes human herpes simplex virus, Chlamydia trachomatis and Adenovirus for external ocular infections and HSV, CMV, VZV for viral retinitis, gram positive, gram negative bacteria, Propionibacterium acnes and fungal genome for endophthalmitis, Mycobacterium tuberculosis, Mycobacterium fortuitum and Mycobacterium chelonae and Toxoplasma gondii genome in for uveitis. Internal control for Human beta globin is also incorporated to check for inhibition of the amplification. The hybridization reaction was later made as a DNA macro- chip in collaboration with X-Cyton, Bangalore. Clinical validation of the was carried out by L & T Microbiology Research Centre, Chennai. The use of the kit does not require Ultraviolet radiation or Radiographic or Spectrophotometric methods. The detection is done by naked eye. The kit was launched on Saturday 22nd September 2007 by our beloved Chairman Emeritus, Sankara Nethralaya, Dr. S. S. Badrinath and Dr. T. Ramasami, Secretary, Department of Science and Technology.



III. DEPARTMENT OF SCIENCE AND TECHNOLOGY (DST)

TITLE: Standardization of nucleic acid based amplification techniques in detection and identification of fungi in clinical specimens from clinically suspected cases of infectious keratitis and endophthalmitis ( SR/SO/HS-63/2003)


OBJECTIVES:

To optimize and apply a multiplex Polymerase Chain reaction (mPCR) targeting 18SrRNA, ITS and 28SrRNA for detection of Panfungal genome from clinically suspected cases of infectious keratitis and endophthalmitis.
To identify the non-sporulating moulds (NSM) by PCR based DNA sequencing and PCR-RFLP.
To apply PCR, PCR based Restriction Fragment length Polymorphism (PCR-RFLP) and DNA sequencing to identify Fusarium species.

I. Optimization of mPCR: mPCR was optimized targeting the 18SrRNA, ITS and 28SrRNA region. The sensitivity of mPCR was 1 picogram and it was specific only the for fungal DNA.. mPCR detected the presence of panfungal genome in 10 corneal scrapings and 10 intraocular fluids in 40 ocular specimens processed.



II. PCR-RFLPand DNA sequencing to identify Fusarium species.Ten isolates of Fusarium species were identified based on colony morphology, microscopic morphology and PCR-RFLP targeting ITS region.



III. PCR based RFLP and DNA sequencing targeting ITS region for the identification of NSM to species level.
A total of 10 NSM isolates were subjected to DNA sequencing. The results of DNA sequencing identified 5 isolates as Botryosphaeria rhodina, 2 as Pythium insidiosum, 1 Macrophomina phaseolina, 1 Rhizoctonia bataticola. The 3 genera consisting of Botryosphaeria, Macrophomina, Rhizoctonia were found to be the potential aetiological agents causing fungal keratitis in Indian population. The 5 isolates of Botryosphaeria rhodina recovered from the same patient exhibited the same type of nucleotide polymorphisms in the ITS region.


Current Eye Research, 2008


IV. DEPARTMENT OF BIOTECHNOLOGY (DBT)

TITLE: Phylogenetic analysis of Human Herpes Simplex Virus (HSV) isolates based on DNA sequencing of genes coding for glycoproteins G, I and E, C regions and to determine novel targets to identify the genotypes of HSV – Funded by DBT (Project No. : DBT/PR9975/MED/29/54/2007) (July 2008 - August 2010)

SUMMARY AND CONCLUSIONS:Phylogenetic analyses of envelope glycoprotein genes HSV provided insight into the existence of genotypes circulating in our population.

Glycoprotein G of HSV1 which did not split into geno-groups by PCR based RFLP as described previously for European strains (mentioned in first year annual report) split into 3 clades, with each clade bearing strains with common variations throwing possibilities of genotypes which are different from European strains.

Glycoprotein I-1 on the other hand, conformed to the European classification, but the VRF strains described as Genotype A, were not seen closely associated, hence showing the possible existence of sub genotypes within genotype A region. Genotype C was not encountered in this study.

Glycoprotein C-2 showed the existence of geno groups, with four distinct clades separating out.

Phylogenetic analyses of Glycoprotein I -2 proved existence of genotypes in an earlier thought conserved genome.

Phylograms of glycoprotein genes E of HSV1 and HSV2 and gC of HSV1 were highly divergent showing them to be rapidly evolving.

Analyses of the thymidine kinase regions showed the existence of 1 resistant strain (HSV2 isolate - vesicle swab) among the fifty tested.

HSV1 ocular isolates 922 and 1105 were seen to be associated closely in the Unique Short region and thymidine kinase region.


BOOKS PUBLISHED:

  1. A Monograph on “Human Cytomegalovirus Molecular Methods For Diagnosis And Study Of Strain Variations” by Dr. H. N. Madhavan and Dr. P. Sowmyapublished by LAP LAMBERT Academic, UK, 2010.
CHAPTER IN A BOOK PUBLISHED


  1. A chapter on Rubella virus “Molecular Methods for Identification of Viruses” by Dr. H. N. Madhavan and Dr. J. Malathi for the book published by CRC Press Taylor & Francis Group Boca Raton, London, New York, 2010

  2. A chapter on Exserohilum “Molecular Methods for Identification of fungi” by Dr. K. Lily Therese & Dr. H. N. Madhavanfor the book published by CRC Press Taylor & Francis Group Boca Raton, London, New York, 2010

  3. A chapter on “An overview on Molecular Diagnostics in Infectious Diseases of the eye - Our experience in Sankara Nethralaya” by Dr. H. N. Madhavan, Dr. K. Lily Therese and Dr. J. Malathi for the “Proceedings of Diagnostics in Infections” Edited by Mr. D. Raghunath and Mr. V. Nagaraja - The Eleventh Sir Dorabji Tata Symposium.Year 2010.

  4. A Chapter on “Application of Polymerase Chain Reaction (PCR) and PCR based methods targeting internal transcribed (ITS) for detection and species level identification of fungi” written by Therese KL, Bagyalakshmi R, Madhavan HN.in the book “Laboratory protocols in Fungal Biology: Current methods in Fungal Biology” Springer UK. March 2012.

  5. A Chapter on “Molecular biological techniques for detection of multidrug resistant tuberculosis (MDR) and extremely drug resistant tuberculosis (XDR) in clinical isolates of Mycobacterium tuberculosis”byTherese KL, Gayathri R, Madhavan HN in the book "Understanding Tuberculosis- Global Experiences and innovative approaches to the Diagnosis” Edited by Pere-Joan Cardona, published byInTech open access, Croatia 2012.

  6. Laboratory Manual Chapter 4 Clinical Microbiology and Serology



PUBLICATIONS(1992 - 2022 - March)
PUBLICATIONS IN PEER REVIEWED JOURNALS: 273

PUBLICATIONS IN THE PEER REVIEWED SCIENTIFIC JOURNALS

2022
  1. Agarwal S, Pandey S, Srinivasan B, Anand AR, Iyer G. Possible Synergistic Role of Cryo-Alcohol Therapy in Infectious Scleritis—Scope and Rationale for Expanding Indications and Review of the Literature. Cornea. 2022 Mar 5.

  2. Jeyalatha MV, Therese KL, Anand AR. An Update on the Laboratory Diagnosis of Neuromyelitis Optica Spectrum Disorders. Journal of Clinical Neurology. 2022 Mar 1;18(2):152-62.

  3. Dhiman R, Agarwal S, Anand AR, Lakshmipathy D, Srinivasan B, Iyer G. Coexistence of Fungal Keratitis in Bilateral Sequential Microsporidial Keratitis–A Rare Case Presentation. Ocular Immunology and Inflammation. 2022 Feb 1:1-3.

  4. Sheerin AA, Anand AR, Mukherjee B. Kocuria rhizophila dacryocystitis: Report of a rare causative organism in a common clinical condition. TNOA Journal of Ophthalmic Science and Research. 2022 Jan 1;60(1):57.

2021
  1. Agarwal M, Asokan R, Therese KL, Lakshmipathy M. Bilateral Acanthamoeba keratitis with radial keratoneuritis–utility of AS-OCT in management and treatment. Clin Exp Optom. 2021 Mar 2:1-3.

  2. Agarwal M, Patnaik G, Agarwal S, Iyer G, Anand AR, Ar G, Biswas J, Zierhut M. Tuberculous Scleritis and Multidrug Resistance. Ocul Immunol Inflamm. 2021 Jan 8:1-10.

  3. Ankit Anil Harwani, Meena Lakshmipathy, K Lily Therese, Jyotirmay Biswas “Bilateral microsporidial stromal keratitis” Indian Journal of Ophthalmology 2021:1:2:190-191

  4. Chandran VA, Koka K, Therese L, Mukherjee B. A Rare Fungal Orbital Infection in an Immunocompetent Young Male Caused by Lichtheimia corymbifera (Absidia corymbifera). Ocular Immunology and Inflammation. 2021 May 23:1-4.

  5. Lakshmipathy D, Janani MK, Madhavan HN.Genotypic identification of Non Tuberculous Mycobacteria from culture proven isolatesA 7 years retrospective study from a Referral laboratory, Chennai. International Journal of Medical Science and Current Research. 2021;4(3):238-24

  6. Gondhale H, Jaichandran VV, Jambulingam M, Anand AR, Srinivasan S, Raman R, Sharma T. Distribution and risk factors of postoperative endophthalmitis in people with diabetes. Indian J Ophthalmol. 2021 Nov;69(11):3329-3334. doi: 10.4103

  7. Das T, Agarwal M, Anand AR, Behera UC, Bhende M, Das AV, Dasgupta D, Dave VP, Gandhi J, Gunasekaran R, Joseph J. Fungal endophthalmitis: Analysis of 730 consecutive eyes from seven tertiary eye care centers in India. Ophthalmology Retina. 2021 Sep 20.

  8. M Agarwal, AR Anand, M Lakshmipathy. Corneal ring infiltrate in fungal keratitis Indian Journal of Ophthalmology-Case Reports 2021; 1 (3), 391

  9. A Nag, A Verma, AR Anand Acute endophthalmitis caused by Ochrobactrum anthropi following cataract surgery in an immunocompetent patient–A case report. Indian Journal of Ophthalmology-Case Reports 2021; 1 (3), 554

  10. Lakshmipathy D, Janani MK, Madhavan HN.Genotypic identification of Non Tuberculous Mycobacteria from culture proven isolatesA 7 years retrospective study from a Referral laboratory, Chennai. International Journal of Medical Science and Current Research. 2021;4(3):238-24

  11. Anand AR, Biswas J. TB or NTM: Can a new multiplex PCR assay be the answer? EBioMedicine. 2021 Aug 26;71:103552. doi: 10.1016/j.ebiom.2021.103552.

  12. Pradhan A, Lakshmipathy D, Biswas J. Polymerase Chain Reaction in Ocular and Adnexal Inflammation: Our Experience with Review of Literature. TNOA J Ophthalmic Sci Res 2021; 59:331-7.

2020
  1. Das T, Agarwal M, Behera U, Bhattacharjee H, Bhende M, Das AV, Dave VP, Dogra A, Ghosh AK, Giridhar S, Joseph J, KaustubhKandle, RoshniKaroliya, PrajnaLalitha, AvinashPathengay, Savitri Sharma &Lily Therese. Diagnosis and management of fungal endophthalmitis: India perspective. Expert Review of Ophthalmology. 2020 Nov 1;15(6):355-65.

  2. Therese KL, Lakshmipathy M, Lakshmipathy D. First report of Mortierellawolfii causing fungal keratitis from a tertiary eye hospital in India. Indian J Ophthalmol. 2020 Oct;68(10):2272.

  3. Nair N, Sudharshan S, Anand AR, Biswas J, Therese KL. Utility of Treponemal Testing from Aqueous Fluid in the Diagnosis of Ocular Syphilis in Patients with HIV/AIDS. OculImmunolInflamm. 2020 Oct 3:1-7.

  4. Agarwal S, Srinivasan B, Janakiraman N, Therese LK, KrishnaKumar S, Patel N, Thenmozhi V, Iyer G. Role of topical ethanol in the treatment of Pythiuminsidiosum keratitis—a proof of concept. Cornea. 2020 Sep 1;39(9):1102-7.

  5. Agarwal S, Srinivasan B, Janakiraman N, Therese LK,KrishnaKumar S, Patel N, Thenmozhi V, Iyer G. Role of topical ethanol in the treatment of Pythiuminsidiosum keratitis—a proof of concept. Cornea2020 Sep 1;39(9):1102-7.

  6. Agarwal M, Patnaik G, Sanghvi K, Anand AR,Janani MK, Biswas J. Clinicopathological, Microbiological and Polymerase Chain Reaction Study in a Case of NocardiaScleritis. OculImmunolInflamm. 2020 Jul 7:1-5. doi: 10.1080/09273948.2020.1770299.

  7. Suganeswari G, Shah D, Anand AR.Intravitrealpiperacillin-tazobactam in endophthalmitis caused by Mycobacterium abscessusin silicone filled eye: A case report. Indian J Ophthalmol. 2020 Jul;68(7):1471-1473.

  8. Jeyalatha Mani V, Parthasarathy D, Padmanabhan P, Narayanan N, Lakshmipathy M, Pachayappan SK, Jayavel P, Therese KL, Rao Madhavan HN, Jambulingam M. Therapeutic Effect of Corneal Crosslinking on Fungal Keratitis: Efficacy of Corneal Collagen Crosslinking as an Adjuvant Therapy for Fungal Keratitis in a Tertiary Eye Hospital in South India. OculImmunolInflamm. 2020 Jun 21:1-8.

  9. Kulandai LT, Lakshmipathy D, Sargunam J. Novel Duplex Polymerase Chain Reaction for the Rapid Detection of Pythiuminsidiosum Directly From Corneal Specimens of Patients With Ocular Pythiosis.Cornea. 2020 Jun;39(6):775-778.

  10. Chatterjee A, Nagarajan H, Padmanabhan P, Vetrivel U, Therese KL,Janakiraman N. Understanding the Uptake Mechanism and Interaction Potential of the Designed Peptide and Preparation of Composite Fiber Matrix for Fungal Keratitis. ACS Omega. 2020 May 21;5(21):12090-102.

  11. Rammohan R, Hajib Naraharirao M, Veerappan S, Vijayaraghavan P, Rajaraman R, Manayath GJ, Dsouza P, Radhakrishnan S, Venkatapathy N, Lakshmipathi D, Madhuravasal Krishnan J,Raghavan A.. Cluster Postoperative Endophthalmitis Caused by Acanthamoeba T10 Genotype—A First Report. Cornea. 2021; 40 ( 2 ): 232-241

  12. KharelSitaula R, Khatri A, Janani MK,Mandage R, Sadhu S, Madhavan HN, Upadhyay MP, Biswas J.  Unfolding COVID-19: Lessons in Learning in Ophthalmology. Clinical Ophthalmology, 2020;14:2807-2820.

  13. Latif N, Janani MK, Selvamuthu P, Majumder PD. Triple trouble: A case of retinochoroiditis in a patient with syphilis, tuberculosis, and human immunodeficiency virus infection. Indian Journal of Ophthalmology. 2020 Sep 1;68(9):1995. (National)

  14. Mahesh M, Sudharshan S, Khetan V, Janani MK, Krishnakumar S. Polymerase chain reaction-proven tuberculous anterior segment mass mimicking juvenile xanthogranuloma in a child. Indian Journal of Ophthalmology. 2020 Sep 1;68(9):2033. (National)

  15. Latiff N, Lakshmipathy M, Janani MK, Majumder PD. Tuberculous corneal ulcer with hypopyon: A case report. Indian Journal of Ophthalmology. 2020 May 1;68(5):922.

  16. Raghavan A, Veerappan S, Rangarajan V, Rajaraman R, Salian R, Mk J, L D, Ganesh VR, Venkatapathy N, Rammohan R.Fulminant Acanthamoeba endolphthalmitis after cataract surgery- Case report. Cornea. 2020; 39(8):1055-1058

2019
  1. Kulandhai LT, Bhagavathi H, Krishnan S, Krishnan K, Balasubramaniyam M, Madhavan HN. Seroprevalence of Anti-HIV-1, Anti-HIV-2, Hepatitis B Surface Antigen, and Anti-HCV in Eye Donors in a Tertiary Eye Hospital, Chennai, India, in the Past 13 years (2005–2017). Cornea. 2019 Jun 1;38(6):737-41.

  2. Amit C, Muralikumar S, Janaki S, Lakshmipathy M, Therese KL, Umashankar V, Padmanabhan P, Narayanan J. Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system. International journal of nanomedicine. 2019;14:605.

  3. Anand AR, Rachel G, Parthasarathy D. HIV proteins and endothelial dysfunction: implications in cardiovascular disease. Frontiers in Cardiovascular Medicine. 2018 Dec 19;5:185.

2018
  1. Amit C, Shalani M, Sargunam J, Meena L, Lily Therese K, Umasankar V, Prema P, Narayanan J. Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system. International Journal of Nanomedicine 2018:13; 1–18.

  2. Anand AR, Rachel G, Parthasarathy D. HIV proteins and endothelial dysfunction: implications in cardiovascular disease. Frontiers in Cardiovascular Medicine. 2018;5:185.

  3. Naik AU, Prakash VJ, Susvar P, Therese KL, Parameswari CK. Postoperative endophthalmitis due to Pseudomonas luteola: First reported case of acute and virulent presentation from a tertiary eye care center in South India. Indian journal of ophthalmology. 2018 Aug;66(8):1200.

  4. Nandagopal M, Parthasarathy D, Mahadevan R, Jayavel P, Jambulingam M. Meta-genomics study among four contact lens wearers from India. Gene Reports. 2018 Sep 1;12:208-17.

  5. Kamal R, Shah D, Sharma S, Janani MK, Kar A, Saurabh K, Roy R, Madhavan HN. Culture-positiveunilateral panophthalmitis in a serology-positive case of dengue hemorrhagic fever. Indian journal of ophthalmology. 2018 Jul;66(7):1017.

  6. Sethi A, Bassi ST, Noronha V, Therese KL. Diagnosis in a case total ophthalmoplegia–where time is life. Neuro-Ophthalmology. 2018 Jun 28:1-3.

  7. Dutta Majumder P, Mukherjee M, Therese KL, Gopal L, Biswas J. Diagnostic Challenge with Nocardia Subretinal Abscess: A Case Report from Tuberculosis-Endemic Region. Ocular immunology and inflammation. 2018 May 12:1-4.

  8. Narayanan N, Vaidehi D, Dhanurekha L, Therese KL, Rajagopal R, Natarajan R, Lakshmipathy M. Unusual ulcerative keratitis caused by Prototheca wickerhamii in a diabetic patient. Indian journal of ophthalmology. 2018 Feb;66(2):311.

  9. Ismail, A. M., Cui, T., Dommaraju, K., Singh, G., Dehghan, S., Seto, J., Shrivastava, S., Fedorova, N. B., Gupta, N., Stockwell, T. B., Halpin, R., Madupu, R., Heim, A., Kajon, A. E., Romanowski, E. G., Kowalski, R. P., Malathi, J., Therese, K. L., Madhavan, H. N., Zhang, Q., Ferreyra, L. J., Jones, M. S., Rajaiya, J., Dyer, D. W., Chodosh, J., … Seto, D. Genomic analysis of a large set of currently-and historically-important human adenovirus pathogens. Emerging microbes & infections.2018 Feb:7(1), 10.

  10. Kharel R, Janani MK, Madhavan HN, Biswas J. Outcome of polymerase chain reaction (PCR) analysis in 100 suspected cases of infectious uveitis. Journal of ophthalmic inflammation and infection. 2018 Dec;8(1):2.

  11. Parthasarathy D, Madhuravasal JK, Jayavel P, Kulandai LT, Rao MH, Jambulingam M. Expression analysis of toll-like receptors of Dengue-infected cornea by real-time polymerase chain reaction. Inflammation Research. 2018 Apr 9:1-4.

  12. Kamal R, Shah D, Sharma S, Janani MK, Kar A, Saurabh K, Roy R, Madhavan HN. Culture-positiveunilateral panophthalmitis in a serology-positive case of dengue hemorrhagic fever. Indian journal of ophthalmology. 2018 Jul;66(7):1017.

  13. Janani MK, Jaichandran V, Madhavan HN, Vijaya L, George RJ, Ambastha PS, Manu A, Malathi J. Effect of Lignocaine Concentration on Human Fibroblasts Growth in Eyes Undergoing Trabeculectomy: An in vitro Study. Biomedicine Hub. 2018;3(3):1-0.

  14. Selvaraj JR, Sudharshan S, Therese LK, Janani MK, Selvamuthu P, Rewri P, Biswas J. Real-time polymerase chain reaction for diagnosis and management of HIV-induced uveitis. Indian journal of ophthalmology. 2018 Nov;66(11):1634.

  15. Janani MK, Durgadevi P, Padmapriya J, Malathi J, Kulandai LT, Madhavan HN. First Report on Detection of Dengue Virus in the Donor Cornea. Cornea. 2018 1;37(12):1586-9

  16. Ekta R, Pukhraj R, PramodBhende, Rajiv R, ParveenS, Pradeep S, Chetan R, Lily TK, Rajshri H. Enterococcus faecalis Endophthalmitis in Children – A 21 Year Study. Ocular Immunology and Inflammation, 2018;26:4, 543 – 549.

  17. Agarwal S, Iyer G, Srinivasan B, Agarwal M, Kumar SP, Therese LK. Clinical profile of pythium keratitis: perioperative measures to reduce risk of recurrence. British Journal of Ophthalmology. 2018 Feb 1;102(2):153-7.

  18. Agarwal S, Iyer G, Srinivasan B, Benurwar S, Agarwal M, Narayanan N, Lakshmipathy M, Radhika N, RajagopalR,KrishnakumarS. K.LilyTherese Clinical profile, risk factors and outcome of medical, surgical and adjunct interventions in patients with Pythium insidiosum keratitis. British Journal of Ophthalmology. September 2018;1–5.

  19. Murugan N, Malathi J, Therese KL, Madhavan HN. Application of six multiplex PCR's among 200 clinical isolates of Pseudomonas aeruginosa for the detection of 20 drug resistance encoding genes. The Kaohsiung journal of medical sciences. 2018 Feb 1;34(2):79-88.

2017
  1. Murugan N, Malathi J, Umashankar V, Madhavan HN. Virulence genome analysis of Pseudomonas aeruginosa VRFPA10 recovered from patient with scleritis. Genomics data. 2017 Jun 30;12:1-3.

  2. Janani MK, Malathi J, , Madhavan HN. Novel Immune Regulatory Pathway Associated with Epstein Barr Virus and Recombinant EBV Protein Mediated Inflammation in Retinal Pigment Epithelium. Annals of Virology and Research. 2017 3(2): 1031.

  3. Verma R, Lee BW, Alameddine RM, Ko AC, Khanna PC, Kikkawa DO, Korn BS Orbital Perivenous Abscess Complicating the Diagnosis and Management of Orbital Cellulitis. Ophthalmic plastic and reconstructive surgery. 2017 Nov 1;33(6):e152-4.

  4. Bhende M, Karpe A, Arunachalam S, Therese KL, Biswas J. Endogenous endophthalmitis due to Roseomonas mucosa presenting as a subretinal abscess. Journal of ophthalmic inflammation and infection. 2017 Dec 1;7(1):5.(International)

  5. Janani MK, Malathi J, , Madhavan HN. Novel Immune Regulatory Pathway Associated with Epstein Barr Virus and Recombinant EBV Protein Mediated Inflammation in Retinal Pigment Epithelium. Annals of Virology and Research. 2017 3(2): 1031.

  6. Shahid Alam, D. Vaidehi, K. Lily Therese, and Mohammad Javed Ali, Rare Pleurostomophora richardsiae Mass Causing Transient Nasolacrimal Duct Obstruction Ophthal Plast Reconstr Surg, April 2017 DOI: 10.1097/IOP.0000000000000919

2016
  1. Jeyalatha MV, Malathi J, Madhavan HN. Stepwise Protocol for Cytospin-enhanced Smearing for Scraped Corneal Epithelial Cells. Applied immunohistochemistry & molecular morphology. 2016 Jan 1;24(1):71-3.

  2. Vetrivel U, Muralikumar S, Mahalakshmi B, Lily KT, Madhavan HN, Alameen M, Thirumudi I. Multilevel Precision-Based Rational Design of Chemical Inhibitors Targeting the Hydrophobic Cleft of Toxoplasma gondii Apical Membrane Antigen 1 (AMA1). Genomics & informatics. 2016 Jun;14(2):53-61.

  3. Murugan N, Malathi J, Umashankar V, Madhavan HN. Resistome and pathogenomics of multidrug resistant (MDR) Pseudomonas aeruginosa VRFPA03, VRFPA05 recovered from alkaline chemical keratitis and post-operative endophthalmitis patient. Gene. 2016 Mar 1;578(1):105-11.578(1), 105-111.

  4. Murugan N, Malathi J, Umashankar V, Madhavan HN. First complete genome sequence of ocular isolate carrying blaVim-2 mediated multi drug resistant (MDR) Pseudomonas aeruginosa VRFPA04 from India and two draft genome sequence of ocular isolates Pseudomonas aeruginosa VRFPA03 and VRFPA05 harboring novel blaDim-1 and blaGes-9 respectively. Int J Pharm Pharm Sci. 2015;12.

  5. Sharma H, Sudharshan S, Therese L, Agarwal M, Biswas J. Candida albicans scleral abscess in a HIV-positive patient and its successful resolution with antifungal therapy—a first case report. Journal of ophthalmic inflammation and infection. 2016 Dec 1;6(1):24. (International)

  6. Raman R, Singh S, Appanraj R, Jambulingam M, Bhende M, Roy R, Sharma T. Five-year incidence and visual outcomes in postintravitreal injection endophthalmitis. Ophthalmology. 2016 May 1;123(5):1162-4.

  7. Agarkar S, Desai R, Jambulingam M, Sumeer SH, Raman R. Incidence, management, and visual outcomes in pediatric endophthalmitis following cataract surgery by a single surgeon. Journal of American Association for Pediatric Ophthalmology and Strabismus. 2016 Oct 1;20(5):415-8.

  8. Ambika S, Balasubramanian M, Theresa L, Veeraputhiran A, Arjundas D. Aquaporin 4 antibody [NMO Ab] status in patients with severe optic neuritis in India. International ophthalmology. 2015 Dec 1;35(6):801-6.

  9. Malathi J, Janani MK, Murugan N. Draft genome sequence of human herpes virus-4 VRF_EBV_01, an epsteinbarr virus obtained from a pediatric post transplantlymphoproliferative disorder (PTLD) patient. International Journal of Pharmacy and Pharmaceutical Sciences. 2015; 62:513-514.

  10. Janani MK, Malathi J, Rela M, Farouk M, Padmapriya J, Madhavan HN. Genotypic detection of Epstein-Barr Virus in pediatric transplant recipients from India. Indian pediatrics. 2015 Nov 1;52(11):946-50.

  11. Rajalakshmy AR, Malathi J, Madhavan HN. Hepatitis C Virus NS3 Mediated Microglial Inflammation via TLR2/TLR6 MyD88/NF-κB Pathway and Toll Like Receptor Ligand Treatment Furnished Immune Tolerance. PloS one. 2015 May 12;10(5):e0125419.

  12. Janani MK, Malathi J, Biswas J, Sridharan S, Madhavan HN. Genotypic detection of epsteinbarr virus from clinically suspected viral retinitis patients in a Tertiary Eye Care Centre, India. Ocular immunology and inflammation. 2015 Sep 3;23(5):384-91.

  13. Sowmiya M, Malathi J, Swarnali S, Priya JP, Therese KL, Madhavan HN. A study on the characterization of Propionibacterium acnes isolated from ocular clinical specimens. The Indian journal of medical research. 2015 Oct;142(4):438.

  14. Janani MK, Jeyalatha MV, Padmanaban P, Madhavan HN, Malathi J. Outbreak of epidemic keratoconjunctivitis caused by human adenovirus type 2 in Chennai, India in 2014. Austin J ClinOphthalmol. 2015;2(4):1054.

  15. Janani MK, Malathi J, Rela M, Farouk M, Padmapriya J, Madhavan HN. Genotypic detection of Epstein-Barr Virus in pediatric transplant recipients from India. Indian pediatrics. 2015 Nov 1;52(11):946-50.

  16. Murugan N, Malathi J, Umashankar V, Madhavan HN. Draft genome sequence of blaVeb-1, blaoxa-10producing multi-drug resistant (MDR) Pseudomonas aeruginosastrain VRFPA09 recovered from bloodstream infection. Brazilian Journal of Microbiology. 2015 Sep;46(3):639-40.

  17. Murugan N, Malathi J, Santhosh S, Lily Therese K, Madhavan HN. Evaluation of a method for detection of both MBL and efflux mechanisms using CCCP±combination method among Pseudomonas aeruginosa isolated from clinical specimens. International Journal of Pharmacy and Pharmaceutical sciences. 2015 Jun;7(8): 185-189.

  18. Murugan N, Malathi J, Umashankar V, Madhavan HN. First Complete Genome Sequence of Ocular Isolate Carrying blaVim-2 mediated Multi-Drug-Resistant(MDR) Pseudomonas aeruginosa VRFPA04 from India and Two Draft genome Sequence of Ocular Isolates Pseudomonas aeruginosa VRFPA03 and VRFPA05. International Journal of Pharmacy and Pharmaceutical sciences. 2015 Oct 7(12): 291-292

  19. Lakshmipathy D, Kulandai LT, Ramasubban G, Rao MH, Rathinam S, Narasimhan M. Nested reverse transcriptase–polymerase chain reactions targeting the messenger RNA of icl2, hspx, and rRNAP1 genes to detect viable Mycobacterium tuberculosis directly from clinical specimens. International journal of mycobacteriology. 2015 Dec 1;4(4):312-7.

  20. Madhavan HN, Bagyalakshmi R, Aarthi P, Ashok C, Prakash A, Sowmiya M, Ninan B, Revathy M. A Study in Neonatal Sepsis Using Polymerase Chain Reaction-Based DNA Sequencing to Detect the Aetiological Agent in the Peripheral Blood of the Neonates with Sepsis. Int. J. Curr. Microbiol. App. Sci. 2015;4(9):415-27.

  21. Thirumudi I, Vetrivel U, Mahalakshmi B, K LT HM. Insights on drug targeting of Toxoplasma gondii host invasion proteins: a review. Asian J Pharm Clin Res. 2015;8(8):52-7.

  22. Verma A, Biswas J, Dhanurekha L, Gayathri R, Therese KL. Detection of Mycobacterium tuberculosis with nested polymerase chain reaction analysis in enucleated eye ball in Eales’ disease. International ophthalmology. 2016 Jun 1;36(3):413-7.

  23. Gondchawar A, Biswas J, Murali S, Eawaran L. A case of herpes simplex keratouveitis diagnosed by real time polymerase chain reaction. Indian journal of ophthalmology. 2015 Nov 1;63(11).

  24. Janani MK, Malathi J, Biswas J, Sridharan S, Madhavan HN. Genotypic detection of epsteinbarr virus from clinically suspected viral retinitis patients in a Tertiary Eye Care Centre, India. Ocular immunology and inflammation. 2015 Sep 3;23(5):384-91.

    25. 2014
  25. Mani Revathy KL, Bagyalakshmi R, Chandrasekar C, Kumar S, Madhavan HN. Application of real time polymerase chain reaction targeting kex 1 gene & its comparison with the conventional methods for rapid detection of Pneumocystis jirovecii in clinical specimens. The Indian journal of medical research. 2014 Sep;140(3):406.

  26. Rajalakshmy AR, Malathi J, Madhavan HN, Srinivasan B, Iyer GK. Hepatitis C virus core and NS3 antigens induced conjunctival inflammation via toll-like receptor–mediated signaling. Molecular vision. 2014;20:1388 -1397

  27. Janani MK, Malathi J, Biswas J, Sridharan S, Madhavan HN. A Correlative Study on Epstein Barr Virus Load Determined by Real Time Pcr with Cytokine Profiles in Pediatric Post Transplant Patients. International Journal of Scientific Research. 2014 Aug. 3 (8): 17-20.

  28. Rajalakshmy AR, Malathi J, Madhavan HN. HCV core and NS3 proteins mediate toll like receptor induced innate immune response in corneal epithelium. Experimental eye research. 2014 Nov 1;128:117-28.

  29. Jeyalatha V, Malathi J, Padmanabhan P, Madhavan HN. Apoptosis of corneal stromal cells induced by telopeptides: An invitrostudy.OphthalmologyRresearch. 2014:2(6): 40-47

  30. Murugan N, Malathi J, Umashankar V, Madhavan HN. Comparative genomic analysis of multidrug-resistant Pseudomonas aeruginosa clinical isolates VRFPA06 and VRFPA08 with VRFPA07. Genome announcements. 2014 May 1;2(2):e00140-14.

  31. Madhavan HN, Bagyalakshmi R Farewell, Chloramphenicol? Is this True?: A Review. Research and Reviews: Journal Of Microbiology and Biotechnology. 2014 Mar 3(1): 13-25.

  32. Gokhale VV, Therese KL, Bagyalakshmi R, Biswas J. Detection of Escherichia fergusonii by PCR-based DNA sequencing in a case of delayed-onset chronic endophthalmitis after cataract surgery. Journal of Cataract & Refractive Surgery. 2014 Feb 1;40(2):327-30.

  33. Moses S, Jambulingam M, Madhavan HN. A pilot study on expression of toll like receptors (TLRs) in response to herpes simplex virus (HSV) infection in acute retinal pigment epithelial cells (ARPE) cells. Journal of postgraduate medicine. 2014 Jul 1;60(3):243.

  34. DhanurekhaLakshmipathy, GayathriRamasubban, Lily Therese, Sridhar R, Madhavan HN, and Meenakshi N. Extensively Drug Resistant Tuberculosis (XDR-TB) by Phenotypic Drug Susceotibility using BACTEC Micro MGIT Culture System – A pilot study in Hospital based Population in Chennai, India. International Journal of Current Microbiology and Applied Sciences 2014; 3(5): 129-135.

  35. Lakshmipathy D, Ramasubban G, Lily Kulandai LT, Sridhar R, Narahari MH, Meenakshi N. Extensively drug resistant tuberculosis (XDR-TB) by phenotypic drug susceptibility using BACTEC Micro MGIT culture system–A pilot study in hospital based population in Chennai, India. Int J CurrMicrobiolAppl Sci. 2014;3:129-35.

  36. Lakshmipathy D, Vetrivel U, Ramasubban G, Kulandai LT, Madhavan HN, Sridhar R, Meenakshi N. Whole genome sequence of polyresistant Mycobacterium tuberculosis CWCFVRF PRTB 19 sputum isolate from Chennai, India, closely clustering with East African Indian 5 genogroup. Genome announcements. 2014 Aug 28;2(4):e00702-14.

  37. RadhaAnnamalai JB, Sudharshan S, Gayathri R, Lily T, Viswanathan S. Real time polymerase chain reaction (RT-PCR) for Mycobacterium tuberculosis in serpiginous choroiditis-A study of 29 cases. International Journal of Medical Research and Health Sciences. 2014 Jan 1;3(3):530-4.

  38. Kulandai LT, Lakshmipathy D, Ramasubban G, Rao MH. Isolation of Mycobacterium massiliense from a corneal biopsy in India. JMM case reports. 2014 Dec 1;1(4).

  39. Kulandai LT, Lakshmipathy D, Ramasubban G, Vetrivel U, Rao MH, Rathinam S, Narasimhan M. Whole-genome sequencing and mutation analysis of two extensively drug-resistant sputum isolates of Mycobacterium tuberculosis (VRFCWCF XDRTB 232 and VRFCWCF XDRTB 1028) from Chennai, India. Genome announcements. 2014 Dec 24;2(6):e01173-14.

  40. Ramasubban G, Therese KL, Lakshmipathy D, Sridhar R, Meenakshi N, Madhavan HN. Detection of novel and reported mutations in the rpoB, katG and inhA genes in multidrug-resistant tuberculosis isolates: a hospital-based study. Journal of global antimicrobial resistance. 2015 Mar 1;3(1):1-4.

  41. GayathriRamasubban, DhanurekhaLakshmipathy, UmashankarVetrivel, Lily Therese Irudayam, H. N. Madhavan, R. Sridhar, N. Meenakshi. Ramasubban G, Lakshmipathy D, Vetrivel U, Kulandai LT, Madhavan HN, Sridhar R, Meenakshi N. Whole-genome sequencing of streptomycin-resistant Mycobacterium tuberculosis isolate VRFCWCF MRTB 180 reveals novel and potential mutations for resistance. Genome announcements. 2014 Sep;2(5).

  42. Rajalakshmy AR, Malathi J, Madhavan HN, Bhaskar S, Iyer GK. Patients with dry eye without hepatitis C virus infection possess the viral RNA in their tears. Cornea. 2015 Jan 1;34(1):28-31.

  43. Gayathri R, Therese LK, Dhanurekha L, Sridhar R, Meenakshi N, Madhavan HN. A pilot study on the detection of multidrug resistant tuberculosis in hospital based population of Chennai, India. Journal of Medical Microbiology & Diagnosis. 2014 Jan 1;3(4):1.

  44. Rajalakshmy AR, Malathi J, Madhavan HN. HCV core and NS3 proteins mediate toll like receptor induced innate immune response in corneal epithelium. Experimental eye research. 2014 Nov 1;128:117-28.

  45. Mukherjee A, Pramanik S, Das D, Roy R, Therese K L. Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique. Indian J Med Microbiol 2014;32:331-2

    46. 2013
  46. Shabna A, Saranya V, Malathi J, Shenbagarathai R, Madhavan HN. Indigenously produced polyhydroxyalkanoate based co‐polymer as cellular supportive biomaterial. Journal of biomedical materials research Part A. 2014 Oct;102(10):3470-6.

  47. Lakshmipathy D, Ramasubban G, Therese L, Vetrivel U, Sivashanmugam M. In silico analysis of novel mutation ala102pro targeting pncA gene of M. tuberculosis. J ComputSciSyst Biol. 2013;6:083-7.

  48. Jeyalatha V, Jambulingam M, Gupta N, Padmanabhan P, Madhavan HN. Study on polymethylmethacrylate ring in protecting limbal stem cells during collagen cross-linking. Ophthalmic research. 2013;50(2):113-6.

  49. Biswas J, Ravi RK, Naryanasamy A, Kulandai LT, Madhavan HN. Eales' disease-current concepts in diagnosis and management. Journal of ophthalmic inflammation and infection. 2013 Dec;3(1):11.

  50. Sowmiya M, Malathi J, Aarthi P, Vaidehi T, Madhavan H.N. Molecular characterization of flic gene by semi nested PCR for the detection of Salmonella entericaserovartyphi in the peripheral blood of patients from Chennai, India. Internatinal Journal of Pharmacy and BioligoicalScience. 2013;4(2):284-91.

  51. Takayama F, Amikura Y, John S, Dedeepiya VD, Senthilkumar R, Venkataramani G, Cherian KM, Madhavan HN, Green DW, Matsumoto M, Suzuki S. Five-year trend of competitiveness and knowledge-gaining attitude of university students; Evaluation based on an event of continuing health sciences education. Journal of Contemporary Medical Education. 2013;1(2):92-9.

  52. Aarthi P, Bagyalakshmi R, Therese KL, Madhavan HN. Development of a novel reverse transcriptase polymerase chain reaction to determine the gram reaction and viability of bacteria in clinical specimens. Microbiological research. 2013 Oct 1;168(8):497-503.

  53. Aarthi P, Bagyalakshmi R, Mohan KR, Krishna M, Nitin M, Madhavan HN, Kalyani S. First case series of emerging rickettsial neonatal sepsis identified by polymerase chain reaction-based deoxyribonucleic acid sequencing. Indian journal of medical microbiology. 2013 Oct 1;31(4):343

  54. Shanthi M, Sekar U, Sowmiya M, Malathi J, Kamalanathan A, Sekar B, Madhavan HN. Clonal diversity of New Delhi metallobetalactamase-1 producing Enterobacteriaceae in a tertiary care centre. Indian journal of medical microbiology. 2013 Jul 1;31(3):237.

  55. Malathi J, Murugan N, Umashankar V, Bagyalakshmi R, Madhavan HN. Draft genome sequence of multidrug-resistant Pseudomonas aeruginosa strain VRFPA02, isolated from a septicemic patient in India. Genome announcements. 2013 Aug 29;1(4):e00425-13.

  56. Therese KL, Gayathri R, Dhanurekha L, Sridhar R, Meenakshi N, Madhavan, HN “Diagnostic appraisal of simultaneous application of two nested PCRs targeting MPB64 gene and IS6110 region for rapid detection of M. tuberculosis genome in culture proven clinical specimens” Indian Journal of Medical Microbiology, 2013 31(4), 366-369.

  57. Samson M, Malathi J, Umashankar V, Sherlin, Madhavan, HN. Novel mutations in the thymidne kinase gene of ocular herps simplex virus isolate responsible for acyclovir resistance by in silico analysis. International Journal of Computational Bioinformatics and In Silico Modeling, 2013 4:159-1662

    58. 2012
  58. Sowmiya M, Malathi J, Madhavan HN. Screening of ocular enterobacteriaceae isolates for presence of chromosomal blaNDM-1 and ESBL genes: a 2-year study at a tertiary eye care center. Investigative ophthalmology & visual science. 2012 Aug 1;53(9):5251-7.

  59. Murali S, Jambulingam M, Tiru V, Kulanthai LT, Rajagopal R, Padmanaban P, Madhavan HN. A study on isolation rate and prevalence of drug resistance among microorganisms isolated from multiorgan donor and donor corneal rim along with a report on existence of bla NDM-1 among Indian population. Current eye research. 2012 Mar 1;37(3):195-203.

  60. Vimalin J, Gupta N, Jambulingam M, Padmanabhan P, Madhavan HN. The effect of riboflavin–UV-A treatment on corneal limbal epithelial cells—a study on human cadaver eyes. Cornea. 2012 Sep 1;31(9):1052-9.

  61. Malathi J, Sowmiya M, Khetan V, Therese KL, Madhavan HN. Complication of Salmonella Bacteremia in a case of treated fungal endophthalmitis. Case reports in ophthalmological medicine. 2012;2012.

  62. Ram S, Vimalin JM, Jambulingam M, Tiru V, Gopalakrishnan RK, Naraharirao MH. Application of PCR-based DNA sequencing technique for the detection of Leptospira in peripheral blood of septicemia patients. Malaysian Journal of Microbiology. 2012 Jan 1;8(1):26-33.

  63. Sudharshan S, Ganesh SK, Balu G, Mahalakshmi B, Therese LK, Madhavan HN, Biswas J. Utility of QuantiFERON®-TB Gold test in diagnosis and management of suspected tubercular uveitis in India. International ophthalmology. 2012 Jun 1;32(3):217-23.

  64. Therese KL, Gayathri R, Dhanurekha L, Sridhar R, Meenakshi N, Madhavan HN, Manoj SE, Vinayagam AK. Detection of Mycobacterium tuberculosis directly from sputum specimens & phenotypic drug resistance pattern of M. tuberculosis isolates from suspected tuberculosis patients in Chennai. The Indian journal of medical research. 2012 May;135(5):778.

  65. SowmyaMurali, UmashankarVetrivel, Muthukumaran, Madhavan H N, JambulingamMalathi, “Studies on New Delhi Metallo-Beta-Lactamse-1 producing Acinetobacter baumannii isolated from Donor Swab in a tertiary eye care centre, India and structural analysis of its antibiotic binding interactions”Bioinformation. 2012; 8: 445–452.

  66. Harishankar A, Jambulingam M, Gowrishankar R, Venkatachalam A, Vetrivel U, Ravichandran S, Yesupadam SM, Madhavan HN. Phylogenetic comparison of exonic US4, US7 and UL44 regions of clinical herpes simplex virus type 1 isolates showed lack of association between their anatomic sites of infection and genotypic/sub genotypic classification. Virology journal. 2012 Dec;9(1):65.

  67. Prathiba K, Bagyalakshmi R, Malathi J, Anusha H, Ravichandran R, Chandy G, Madhavan HN. Optimization and application of subtype specific polymerase chain reaction for detection and identification of mixed subtypes of hepatitis C virus in patients with renal disease. Journal of virological methods. 2012 Dec 1;186(1-2):113-8.

  68. Therese KL, Gayathri R, Balasubramanian S, Natrajan S, Madhavan HN. Phenotypic and genotypic characteristics of drug resistance in Mycobacterium tuberculosis isolates from pediatric population of Chennai, India. Indian journal of medical microbiology. 2012 Oct 1;30(4):411.

  69. Janani MK, Malathi J, Madhavan HN. Isolation of a variant human adenovirus identified based on phylogenetic analysis during an outbreak of acute keratoconjunctivitis in Chennai. The Indian journal of medical research. 2012 Aug;136(2):260.

    70. 2011
  70. Therese KL, Gayathri R, Thiruppathi K, Madhavan HN. First report on isolation of Mycobacterium monacense from sputum specimen in India. Lung India: official organ of Indian Chest Society. 2011 Apr;28(2):124

  71. Ramasubban G, Therese KL, Vetrivel U, Sivashanmugam M, Rajan P, Sridhar R, Madhavan HN, Meenakshi N. Detection of novel coupled mutations in the katG gene (His276Met, Gln295His and Ser315Thr) in a multidrug-resistant Mycobacterium tuberculosis strain from Chennai, India, and insight into the molecular mechanism of isoniazid resistance using structural bioinformatics approaches. International journal of antimicrobial agents. 2011 Apr 1;37(4):368-72.

  72. Aarthi P, Harini R, Sowmiya M, Malathi J, Therese KL, Madhavan HN. Identification of bacteria in culture negative and polymerase chain reaction (PCR) positive intraocular specimen from patients with infectious endopthalmitis. Journal of microbiological methods. 2011 Apr 1;85(1):47-52.

  73. Malathi J, Umashankar V, Sathyabaarathi R, Muthukumaran S, Ishwarya M, Madhavan HN. Functional characterization of novel mutations in UL54 of ganciclovir resistant HCMV strain using structural analysis. Bioinformation. 2011;5(9):390.

    74. 2010
  74. Mahalakshmi B, Therese KL, Kirthika R, Madhavan HN, Biswas J, Sudharshan S. Evaluation of nested PCRs targeting the BI and SAG2 genes for detection of Toxoplasma gondii genome in aqueous humor from HIV positive toxoplasma retinochoroiditis patients in a tertiary eye hospital. Current Research in Medicine. 2010;1(2):157-63.

  75. Bagyalakshmi R, Therese KL, Malathi J, Mahalakshmi B, Prasanna S, Madhavan H. Comparative evaluation of Bactec culture using Peds Plus F broth with standard microbiological methods in detection of microbial aetiologies in vitreous aspirates in infectious endophthalmitis. Internet J Ophthalmol Vis Sci. 2010;8(1).

  76. Malathi J, Suresh P, Sagar L, Margarita S, Hajib M. A study on the incidence, microbiological analysis and investigations on the source of infection of postoperative infectious endophthalmitis in a tertiary care ophthalmic hospital & 58; An 8-year study. Indian Journal of Ophthalmology. 2010 Jan 1;58(4):297-302.

  77. Mahalakshmi B, Lily Therese K, Devipriya U, Pushpalatha V, Margarita S, Madhavan HN. Infectious aetiology of congenital cataract based on TORCHES screening in a tertiary eye hospital in Chennai, Tamil Nadu, India. Indian Journal of Medical Research. 2010 Apr 1;131(4):559.

  78. Gayathri R, Therese KL, Deepa P, Mangai S, Madhavan HN. Antibiotic susceptibility pattern of rapidly growing mycobacteria. Journal of postgraduate medicine. 2010 Apr 1;56(2):76.

  79. Nair AG, Fudnawala V, Arjundas D, Noronha VO, Malathi J, Therese L, Gandhi RA. Isolated Unilateral Infiltrative Cryptococcal Optic Neuropathy in an Immunocompetent individual. Neuro-ophthalmology. 2010 Aug 1;34(4):314-8.

  80. Mahendradas P, Shetty R, Malathi J, Madhavan HN. Chikungunya virus iridocyclitis in Fuchs’ heterochromiciridocyclitis. Indian journal of ophthalmology. 2010 Nov;58(6):545.

  81. Madhavan HN, Samson MY, Ishwarya M, Vijayakumar R, Jambulingam M. pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies. Virology journal. 2010 Dec;7(1):322.

    82. 2009
  82. Malathi J, Sowmiya M, Margarita S, Madhavan HN, Therese KL. Application of PCR based--RFLP for species identification of ocular isolates of methicillin resistant staphylococci (MRS). Indian Journal of Medical Research. 2009 Jul 1;130(1):78.

  83. Sowmya P, Madhavan HN. Diagnostic utility of polymerase chain reaction on intraocular specimens to establish the etiology of infectious endophthalmitis. European journal of ophthalmology. 2009 Sep;19(5):812-7.

  84. Sowmya P, Madhavan HN. Analysis of mixed infections by multiple genotypes of human cytomegalovirus in immunocompromised patients. Journal of medical virology. 2009 May;81(5):861-9.

  85. Balasubramanian S, Jasty S, Sitalakshmi G, Madhavan HN, Krishnakumar S. Influence of feeder layer on the expression of stem cell markers in cultured limbal corneal epithelial cells. Indian Journal of Medical Research. 2008 Nov 1;128(5):616.

  86. Therese KL, Bartell J, Deepa P, Mangaiyarkarasi S, Ward D, Dajcs J, Madhavan HN, Stroman D. DNA sequencing by Microseq kit targeting 16S rRNA gene for species level identification of mycobacteria. Indian Journal of Medical Research. 2009 Feb 1;129(2).

  87. Rishi E, Rishi P, Nandi K, Shroff D, Therese KL. Endophthalmitis caused by Enterococcus faecalis: a case series. Retina. 2009 Feb 1;29(2):214-7.

    88. 2008
  88. Bagyalakshmi R, Senthilvelan B, Therese KL, Murugusundram S, Madhavan HN. Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens. Indian journal of dermatology. 2008 Jan;53(1):15.

  89. Bagyalakshmi R, Therese KL, Prasanna S, Madhavan HN, “Newer emerging pathogens of ocular Non sporulating moulds (NSM) identified by Polymerase chain reaction (PCR) based DNA sequencing technique targeting internal transcribed spacer (ITS) region”. Current Eye Research, 2008 33:139-147.

  90. Nandi K, Ranjan P, Therese L, Biswas J. Polymerase chain reaction in intraocular inflammation. The open ophthalmology journal. 2008;2:141.

  91. Sitalakshmi G, Sudha B, Madhavan HN, Vinay S, Krishnakumar S, Mori Y, Yoshioka H, Abraham S. Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model. Tissue Engineering Part A. 2008 Aug 23;15(2):407-15.

  92. Mohan ER, Kabra S, Udhay P, Madhavan HN. Intracanalicular antibiotics may obviate the need for surgical management of chronic suppurative canaliculitis. Indian journal of ophthalmology. 2008 Jul;56(4):338.

  93. Bagyalakshmi R, Therese KL, Prasanna S, Madhavan HN. Newer emerging pathogens of ocular non-sporulating molds (NSM) identified by polymerase chain reaction (PCR)-based DNA sequencing technique targeting internal transcribed spacer (ITS) region. Current eye research. 2008 Jan 1;33(2):139-47.

  94. Shyamala G, Sowmya P, Madhavan HN, Malathi J. Relative efficiency of polymerase chain reaction and enzyme-linked immunosorbant assay in determination of viral etiology in congenital cataract in infants. Journal of postgraduate medicine. 2008 Jan 1;54(1):17.

  95. Biwas J, Bagyalakshmi R, Therese LK. Diagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism. Indian journal of ophthalmology. 2008 Jan;56(1):65.

  96. Therese KL, Bagyalakshmi R, Prasanna S, Madhavan HN, Reply to the Editor Current Eye Research, 2008 33 : 905-6

    97. 2007
  97. Palani D, Kulandai LT, Naraharirao MH, Guruswami S, Ramendra B. Application of polymerase chain reaction-based restriction fragment length polymorphism in typing ocular rapid-growing nontuberculous mycobacterial isolates from three patients with postoperative endophthalmitis. Cornea. 2007 Jul 1;26(6):729-35

  98. Shankar S, Biswas J, Gopal L, Bagyalakshmi R, Therese L, Borse NJ. Anterior chamber exudative mass due to Scedosporiumapiospermum in an immunocompetent individual. Indian journal of ophthalmology. 2007 May 1;55(3):226.

  99. Mahalakshmi B, Therese KL, Shyamala G, Devipriya U, Madhavan HN. Toxoplasma gondii detection by nested polymerase chain reaction in lens aspirate and peripheral blood leukocyte in congenital cataract patients: the first report from a tertiary eye hospital in India. Current eye research. 2007 Jan 1;32(7-8):653-7.

  100. Sowmya P, Dhanya V, Madhavan HN, Therese KL. Comparative efficacy of PCR-based restriction fragment length polymorphism (RFLP) & multiplex PCR for glycoprotein B (gB) genotyping of human cytomegalovirus. Indian Journal of Medical Research. 2007 Aug 1;126(2):122.

  101. Therese KL, Deepa P, Therese J, Bagyalakshmi R, Biswas J, Madhavan HN. Association of mycobacteria with Eales' disease. Indian Journal of Medical Research. 2007 Jul 1;126(1):56.

  102. Dhivya S, Madhavan HN, Rao CM, Rao KS, Ramchander PV, Therese KL, Malathi J. Comparison of a novel semi-nested polymerase chain reaction (PCR) with a uniplex PCR for the detection of Acanthamoeba genome in corneal scrapings. Parasitology research. 2007 May 1;100(6):1303-9.

  103. Madhavan HN, Sowmya P, Therese KL, Malathi J. Development and application of a novel multiplex polymerase chain reaction for semi-quantitation of Human Cytomegalovirus in clinical specimens. Journal of virological methods. 2007 May 1;141(2):166-72.

  104. Sudha B, Madhavan HN, Sitalakshmi G, Malathi J, Krishnakumar S, Mori Y, Yoshioka H, Abraham S. Cultivation of human corneal limbal stem cells in Mebiol gel®-A thermo-reversible gelation polymer. Indian Journal of Medical Research. 2006 Dec 1;124(6):655.

  105. G. Shyamala, J. Malathi, Y.S. Moses, K.L. Therese, H.N. Madhavan. Nested reverse transcription polymerase chain reaction for the detection of rubella virus in clinical specimens. Indian Journal of Medical research 2007; 125:73-78.

  106. Bagyalakshmi R, Therese KL, Madhavan HN. Nucleotide polymorphisms associated with Internal Transcribed Spacer (ITS) regions of ocular isolates of Aspergillus flavus. Journal of microbiological methods. 2007 Jan 1;68(1):1-0.

    107. 2006
  107. Therese KL, Bagyalakshmi R, Madhavan HN, Deepa P. In-vitro susceptibility testing by agar dilution method to determine the minimum inhibitory concentrations of amphotericin B, fluconazole and ketoconazole against ocular fungal isolates. Indian journal of medical microbiology. 2006 Oct 1;24(4):273.

  108. Bagyalakshmi R, Madhavan H, Therese K. Development and application of multiplex polymerase chain reaction for the etiological diagnosis of infectious endophthalmitis. Journal of Postgraduate Medicine. 2006 Jul 1;52(3).

  109. Mahalakshmi B, Therese KL, Madhavan HN, Biswas J. Diagnostic value of specific local antibody production and nucleic acid amplification technique-nested polymerase chain reaction (nPCR) in clinically suspected ocular toxoplasmosis. Ocular immunology and inflammation. 2006 Jan 1;14(2):105-12.

  110. Sowmya P, Madhavan HN, Therese KL. Evaluation of three polymerase chain reaction tests targeting morphological transforming region II, UL-83 gene and glycoprotein O gene for the detection of human cytomegalovirus genome in clinical specimens of immunocompromised patients in Chennai, India. Virology journal. 2006 Dec;3(1):20.

  111. Malathi J, Madhavan HN, Therese KL, Margarita S. Phacoemulsification probe as a source of postoperative endophthalmitis following phacoemulsification cataract extraction (PKE) surgery—DNA sequencing-based study. Journal of Hospital Infection. 2006 Jan 1;62(1):117-9.

  112. Babu RB, Sudharshan S, Kumarasamy N, Therese L, Biswas J. Ocular tuberculosis in acquired immunodeficiency syndrome. American journal of ophthalmology. 2006 Sep 1;142(3):413-8.

  113. Babu RB, Sudharshan S, Kumarasamy N, Therese L, Biswas J. Ocular tuberculosis in acquired immunodeficiency syndrome. American journal of ophthalmology. 2006 Sep 1;142(3):413-8.

    114. 2005
  114. Shyamala G, Sowmya P, Sudha B, Malathi J, Therese LK, Madhavan HN. Application of polymerase chain reaction to differentiate herpes simplex virus 1 and 2 serotypes in culture negative intraocular aspirates. Indian journal of medical microbiology. 2005 Oct 1;23(4):239.

  115. Shyamala G, Sowmya P, Sudha B, Malathi J, Therese LK, Madhavan HN. Application of polymerase chain reaction to differentiate herpes simplex virus 1 and 2 serotypes in culture negative intraocular aspirates. Indian journal of medical microbiology. 2005 Oct 1;23(4):239.

  116. P Deepa, K L Therese, H N Madhavan. Detection and Characterization of mutations in Rifampicin resistant Mycobacterium tuberculosis Clinical isolates by DNA sequencing. Indian Journal of Tuberculosis 2005; 52:132-136

  117. Therese KL, Jayanthi U, Madhavan HN. Application of nested polymerase chain reaction (nPCR) using MPB 64 gene primers to detect Mycobacterium tuberculosis DNA in clinical specimens from extrapulmonary tuberculosis patients. Indian Journal of Medical Research. 2005 Aug 1;122(2):165.

  118. Fogla R, Padmanabhan P, Therese KL, Biswas J, Madhavan HN. Chronic microsporidial stromal keratitis in an immunocompetent, non-contact lens wearer. Indian journal of ophthalmology. 2005 Apr 1;53(2):123.

  119. Deepa P, Therese KL, Madhavan HN. Application of PCR-based restriction fragment length polymorphism (RFLP) for the identification of mycobacterial isolates. Indian Journal of Medical Research. 2005 May 1;121(5):694.

  120. Baskaran M, Rao SK, Kumar PR, Vijaya L, Madhavan HN. Postphacoemulsification endophthalmitis—role of residual debris in the handsets used for surgery. Eye. 2005 Jan;19(1):115.

    121. 2004
  121. Malathi J, Madhavan HN, Therese KL, Shyamala G. Polymerase chain reaction to detect Chlamydia trachomatis and adenovirus in the nasopharyngeal aspirates from paediatric patients with lower respiratory infections. Indian journal of pathology & microbiology. 2004 Apr;47(2):302-5.

  122. Madhavan HN, Malathi J, Joseph PR, Yuichi M, Abraham SJ, Yoshioka H. A study on the growth of continuous culture cell lines embedded in Mebiol gel. Current Science. 2004 Nov 10:1275-7.

  123. Priya K, Mahalakshmi B, Malathi J, Biswas J, Sukumar B, Madhavan HN. Prevalence of herpes simplex virus, varicella zoster virus and cytomegalovirus in HIV-positive and HIV-negative patients with viral retinitis in India. European Journal of Clinical Microbiology & Infectious Diseases. 2004 Nov 20;23(11):857-8.

  124. Malathi J, Shyamala G, Madhavan HN. Relative susceptibility of six continuous cell lines for cultivation of Chlamydia trachomatis strains. Indian journal of medical microbiology. 2004 Jul 1;22(3):169.

  125. Baskaran M, Rao SK, Kumar PR, Vijaya L, Madhavan HN. Postphacoemulsification endophthalmitis—role of residual debris in the handsets used for surgery. Eye. 2005 Jan;19(1):115.

  126. Mahalakshmi B, Madhavan HN, Pushpalatha R, Margarita S. Seroprevalence of human immuno-deficiency virus, hepatitis B virus and hepatitis C virus among eye donors. Indian journal of ophthalmology. 2004 Mar 1;52(1):61.

  127. Rao SK, Baskaran M, Kumar PJ, Vijaya L, Madhavan HN. Debris in phacoemulsification handsets. A potential cause of endophthalmitis after cataract surgery?. Indian journal of ophthalmology. 2004 Mar 1;52(1):80.

  128. Jayanthi U, Madhavan HN, Therese KL. Nucleic acid amplification tests for diagnosis of tuberculous meningitis. The Lancet Infectious Diseases. 2004 Jan 1;4(1):9.

    129. 2003
  129. Gopal L, Ramaswamy AA, Madhavan HN, Saswade M, Battu RR. Postoperative endophthalmitis caused by sequestered Acinetobacter calcoaceticus. American journal of ophthalmology. 2000 Mar 1;129(3):388-90.

  130. Priya K, Madhavan HN, Malathi J. Use of uniplex polymerase chain reaction & evaluation of multiplex PCR in the rapid diagnosis of viral retinitis. Indian Journal of Medical Research. 2003 May 1;117:205-10.

  131. Malathi J, Madhavan HN, Therese KL, Joseph PR, Bao Y. A hospital based study on the prevalence of conjunctivitis due to Chlamydia trachomatis. Indian Journal of Medical Research. 2003 Feb 1;117:71-5.

  132. Madhavan HN, Priya K, Malathi J, Joseph PR. Laboratory methods in the detection of herpes simplex virus (HSV) in keratitis--a 9-year study including polymerase chain reaction (PCR) during last 4 years. Indian journal of pathology & microbiology. 2003 Jan;46(1):109-12

  133. Madhavan HN, Priya K, Bagyalakshmi R. Phenotypic and genotypic methods for the detection of herpes simplex virus serotypes. Journal of virological methods. 2003 Mar 1;108(1):97-102.

  134. Madhavan HN. Laboratory investigations on viral and Chlamydia trachomatis infections of the eye: Sankara Nethralaya experiences. Indian journal of ophthalmology. 1999 Dec 1;47(4):241.

  135. Gopal L, Rao SK, Biswas J, Madhavan HN, Agarwal S. Tuberculous granuloma managed by full thickness eye wall resection. American journal of ophthalmology. 2003 Jan 1;135(1):93-4.

  136. Biswas J, Narayana KM, Gupta S, Malathi J, Madhavan HN. Panuveitis due to acquired rubella and isolation of rubella virus from the aqueous humor. Journal of pediatric ophthalmology and strabismus. 2003 Jul 1;40(4):240-2.

  137. Gopal L, Ramaswamy AA, Madhavan HN, Battu RR, Sharma T, Shanmugam MP, Bhende PS, Bhende M, Ratra D, Shetty NS, Rao MK. Endophthalmitis caused by Acinetobacter calcoaceticus. A profile. Indian journal of ophthalmology. 2003 Dec 1;51(4):335.

    138. 2002
  138. Priya K, Madhavan HN, Reiser BJ, Biswas J, Saptagirish R, Narayana KM, Rao NA. Association of herpesviruses in the aqueous humor of patients with serpiginous choroiditis: a polymerase chain reaction-based study. Ocular immunology and inflammation. 2002 Jan 1;10(4):253-61.

  139. Priya K, Madhavan HN. Diagnostic value of enzyme linked immuno-sorbent assay for cytomegalovirus disease. Journal of postgraduate medicine. 2002 Jul 1;48(3):176.

  140. Malathi J, Madhavan HN, Therese KL, Rinku JP, Narendar KP. Prevalence of Chlamydia trachomatis & herpes simplex virus in males with urethritis & females with cervicitis attending STD clinic. Indian Journal of Medical Research. 2002 Aug 1;116:58.

  141. Madhavan HN, Priya K, Malathi J, Joseph PR. Preparation of amniotic membrane for ocular surface reconstruction. Indian journal of ophthalmology. 2002 Sep 1;50(3):227.

  142. Madhavan HN, Priya K. Polymerase chain reaction based restriction fragment length polymorphism for the genotyping of cytomegalovirus (CMV) from patients with CMV disease in Chennai. Indian Journal of Medical Research. 2002 Jun 1;115:242.

  143. Biswas J, Kumar SK, Rupauliha P, Misra S, Bharadwaj I, Therese L. Detection of mycobacterium tuberculosis by nested polymerase chain reaction in a case of subconjunctival tuberculosis. Cornea. 2002 Jan 1;21(1):123-5.

  144. Biswas J, Fogla R, Gopal L, Narayana KM, Banker AS, Kumarasamy N, Madhavan HN. Current approaches to diagnosis and management of ocular lesions in human immunodeficiency virus positive patients. Indian journal of ophthalmology. 2002 Jun 1;50(2):83.

  145. Wagle AM, Biswas J, Gopal L, Madhavan HN. Clinical profile and immunological status of cytomegalovirus retinitis in organ transplant recipients. Indian journal of ophthalmology. 2002 Jun;50(2):115-21.

  146. Biswas J, Sharma T, Gopal L, Madhavan HN, Sulochana KN, Ramakrishnan S. Eales disease—an update. Survey of ophthalmology. 2002 May 1;47(3):197-214.

  147. Madhavan HN, Rao SK. Ciprofloxacin precipitates in the corneal epithelium. Journal of Cataract & Refractive Surgery. 2002 Jun 1;28(6):909.

  148. Madhavan HN, Therese KL, Doraiswamy K. Further investigations on the association of Mycobacterium tuberculosis with Eales' disease. Indian journal of ophthalmology. 2002 Mar 1;50(1):35.

  149. Narasimhan S, Madhavan HN. Development and application of an in vitro susceptibility test for Acanthamoeba species isolated from keratitis to polyhexamethylenebiguanide and chlorhexidine. Cornea. 2002 Mar 1;21(2):203-5.

  150. Madhavan HN, Goldsmith CS, Rao SK, Fogla R, Malathi J, Priya K. Isolation of a vesicular virus belonging to the family rhabdoviridae from the aqueous humor of a patient with bilateral corneal endotheliitis. Cornea. 2002 Apr 1;21(3):333-5.

  151. Priya K, Madhavan HN. Use of nested polymerase chain reaction for the detection of cytomegalovirus in clinical specimens. Indian Journal of Medical Research. 2002 Jan 1;115:5.

    152. 2001
  152. Malathi J, Therese KL, Madhavan HN. The association of rubella virus in congenital cataract—a hospital-based study in India. Journal of clinical virology. 2001 Dec 1;23(1-2):25-9.

  153. Biswas J, Roy Chowdhury B, Krishna Kumar S, Lily Therese K, Madhavan HN. Detection of Mycobacterium tuberculosis by polymerase chain reaction in a case of orbital tuberculosis. Orbit. 2001 Jan 1;20(1):69-74.

  154. Anand AR, Madhavan HN, Sudha NV, Therese KL. Polymerase chain reaction in the diagnosis of Aspergillus endophthalmitis. Indian Journal of Medical Research. 2001 Oct 1;114:133.

  155. Gunisha P, Anand AR, Therese KL and Madhavan HN. Use of Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (RFLP) to trace the source of Alcaligenes (Achromobacter) xylosoxidans contaminating ocular clinical specimens. Indian Journal of Applied Microbiology, 1: 41-45, 2001.

  156. Fogla R, Rao SK, Anand AR, Madhavan HN. Insect wing case: unusual foreign body. Cornea. 2001 Jan 1;20(1):119-21.

  157. Priya K. Application of rapid aetiological diagnostic methods on intraocular specimens in viral retino-choroiditis. Indian Journal of Medical Microbiology. 2001;19(1):14-9.

  158. Biswas J, Deka S, Padmaja S, Madhavan HN, Kumarasamy N, Solomon S. Central retinal vein occlusion due to herpes zoster as the initial presenting sign in a patient with acquired immunodeficiency syndrome (AIDS). Ocular immunology and inflammation. 2001 Jan 1;9(2):125-30.

  159. Gunisha P, Madhavan HN, Jayanthi U, Therese KL. Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples. Indian journal of pathology & microbiology. 2001 Apr;44(2):97-102.

    160. 2000
  160. Biswas J, Kumar AA, George AE, Madhavan HN, Kumarasamy N, Mothi SN, Solomon S. Ocular and systemic lesions in children with HIV. The Indian Journal of Pediatrics. 2000 Oct 1;67(10):721-4.

  161. Anand AR, Madhavan HN, Therese KL. Use of polymerase chain reaction (PCR) and DNA probe hybridization to determine the Gram reaction of the infecting bacterium in the intraocular fluids of patients with endophthalmitis. The Journal of infection. 2000 Nov;41(3):221-6.

  162. Rao SK, Sharma T, Parikh S, Madhavan HN, Padmanabhan P. Explantation of silicone plate haptic intraocular lenses. Ophthalmic Surgery, Lasers and Imaging Retina. 1999 Jul 1;30(7):575-8.

  163. Ramaswamy AA, Biswas J, Bhaskar V, Gopal L, Rajagopal R, Madhavan HN. Postoperative Mycobacterium chelonae endophthalmitis after extracapsular cataract extraction and posterior chamber intraocular lens implantation. Ophthalmology. 2000 Jul 1;107(7):1283-6.

  164. Madhavan HN. Guidelines for Direct Diagnosis in External Ocular Infections and Inflammations. Journal of Tamil Nadu Ophthalmic Association, 2000, 41: 63-68.

  165. Madhavan H, Malathy J, Priya K. An outbreak of acute conjunctivitis caused by Coxsackie virus A 24. Indian journal of ophthalmology. 2000 Apr 1;48(2).

  166. Therese LK, Priya K, Rao SK, Biswas J, Madhavan HN. Herpes simplex virus DNA in the lens one year after an episode of retinitis. Indian journal of ophthalmology. 2000 Jun 1;48(2):140.

  167. Anand A.R., Therese, K.L. and Madhavan, HN. Spectrum of aetiological agents of Postoperative Endophthalmitis and antibiotic Susceptibility of Bacterial isolates. Indian J Ophthalomol. 2000 48 : 123-28,

  168. Fogla R, Biswas J, Kumar SK, Kumarasamy N, Madhavan HN, Solomon S. Squamous cell carcinoma of the conjunctiva as initial presenting sign in a patient with acquired immunodeficiency syndrome (AIDS) due to human immunodeficiency virus type-2. Eye. 2000 Mar;14(2):246.

  169. Battu RR, Biswas J, Jayakumar N, Madhavan HN, Kumarsamy N, Solomon S. Papilloedema with peripapillary retinal haemorrhages in an acquired immunodeficiency syndrome (AIDS) patient with cryptococcal meningitis. Indian journal of ophthalmology. 2000 Mar 1;48(1):47.

  170. Fogla R, Biswas J, Parikh S, Madhavan HN. Micrococcal endophthalmitis following extracapsular cataract extraction with foldable silicone intraocular lens implantation. Indian journal of ophthalmology. 2000 Mar 1;48(1):50.

  171. Gopal L, Ramaswamy AA, Madhavan HN, Saswade M, Battu RR. Postoperative endophthalmitis caused by sequestered Acinetobacter calcoaceticus. American journal of ophthalmology. 2000 Mar 1;129(3):388-90.

  172. Choudhry S, Rao SK, Biswas J, Madhavan HN. Necrotizing nocardialscleritis with intraocular extension: a case report. Cornea. 2000 Mar 1;19(2):246-8.

  173. Madhavan HN, Therese KL, Gunisha P, Jayanthi U, Biswas J. Polymerase chain reaction for detection of Mycobacterium tuberculosis in epiretinal membrane in Eales’ disease. Investigative ophthalmology & visual science. 2000 Mar 1;41(3):822-5.

  174. Biswas J, Madhavan HN, George AE, Kumarasamy N, Solomon S. Ocular lesions associated with HIV infection in India: a series of 100 consecutive patients evaluated at a referral center. American Journal of Ophthalmology. 2000 Jan 1;129(1):9-15.

  175. Castelino AM, Rao SK, Biswas J, Gopal L, Madhavan HN, Kumar SK. Conjunctival rhinosporidiosis associated with scleral melting and staphyloma formation: diagnosis and management. Cornea. 2000 Jan 1;19(1):30-3.

    176. 1999
  176. Madhavan HN, Priya K, Anand AR, Therese KL. Detection of herpes simplex virus (HSV) genome using polymerase chain reaction (PCR) in clinical samples: comparison of PCR with standard laboratory methods for the detection of HSV. Journal of clinical virology. 1999 Oct 1;14(2):145-51.

  177. Madhavan HN, Anand AR and Therese KL. Infectious Endophthalmitis. Indian J Med Microbiol. 1999; 17: 108-15.

  178. Madhavan HN. Laboratory investigations on viral and Chlamydia trachomatis infections of the eye: Sankara Nethralaya experiences. Indian journal of ophthalmology. 1999 Dec 1;47(4):241.

  179. Madhavan HN, Rao SK, Joseph PR, Sulochana KN. Antibacterial activity of the white precipitate formed on the corneal surface after treatment with ciprofloxacin ophthalmic solution. Cornea. 1999 Sep;18(5):549-52.

  180. Biswas J, Therese L, Madhavan HN. Use of polymerase chain reaction in detection of Mycobacterium tuberculosis complex DNA from vitreous sample of Eales’ disease. British Journal of Ophthalmology. 1999 Aug 1;83(8):994-.

    181. 1998
  181. Therese KL, Anand AR, Madhavan HN. Polymerase chain reaction in the diagnosis of bacterial endophthalmitis. British Journal of Ophthalmology. 1998 Sep 1;82(9):1078-82.

  182. Madhavan HN, Roy S. Effects of viscoelastic ophthalmic solutions on cell cultures. Indian journal of ophthalmology. 1998 Mar 1;46(1):37.

  183. Biswas J, Gopal L, Sharma T, Parikh S, Madhavan HN, Badrinath SS. Recurrent cryptococcal choroiditis in a renal transplant patient: clinicopathologic study. Retina. 1998 Mar 1;18(3):273-6.

  184. Sharma T, Gopal L, Therese L. Endophthalmitis in eyes following vitrectomy. Ophthalmic Surgery, Lasers and Imaging Retina. 1998 Oct 1;29(10):857-9.

  185. Madhavan HN. Conjunctivitis and acute retinal inflammation: a review of virological investigations at sankaranethralaya, Chennai. Indian Journal of Medical Microbiology. 1998 Jan 1;16(1):12

  186. Biswas J, Therese KL and Madhavan HN. Use of polymerase chain reaction (PCR) in the detection of Mycobacterium tuberculosis complex DNA from aqueous sample of suspected tuberculosis uveitis. Uveitis Today: Elsevier Science 1998: 227-9.

  187. Biswas J, Sulochana KN, Ramakrishnan S, Madhavan HN. Eales' disease-Clinical and basic research at Sankara Nethralaya. Uveitis Today: Elsevier Science. 1998:111-4.

  188. Takeuchi S. Surveillance of viral conjunctivitis in Asian countries. Japanese Journal Of Clinical Ophthalmology. 1998;52:513-6.

  189. Biswas J, Mukesh BN, Narain S, Roy S, Madhavan HN. Profiling of human leukocyte antigens in Eales ‘disease. International ophthalmology. 1997 Sep 1;21(5):277-81.

  190. Therese L, Madhavan HN. Anaerobic bacteria associated with endophthalmitis: 5-year prospective study. Annals of ophthalmology. Glaucoma. 1998;30(5):305-8.

  191. Madhavan HN, Biswas A, Malathy J, Mukesh BN. Microbiological assay of Ampicillin in serum and aqueous humor of patients given Ampicillin-Sulbactam injection. Indian journal of ophthalmology. 1998 Jun 1;46(2):97.

  192. Dalapathy S, Lily TK, Roy S, Madhavan HN. Development and use of nested polymerase chain reaction (PCR) for the detection of adenovirus from conjunctivitis specimens. Journal of clinical virology. 1998 Jul 24;11(1):77-84.

    193. 1997
  193. Bhende PS, Biswas J, Madhavan HN. Serological evidence for active Epstein-Barr virus infection in multifocal choroiditis. Indian journal of pathology & microbiology. 1997 Apr;40(2):129-31.

  194. Biswas J, Therese L, Kumarasamy N, Solomon S, Yesudian P. Lid abscess with extensive molluscumcontagiosum in a patient with acquired immunodeficiency syndrome. Indian journal of ophthalmology. 1997 Dec 1;45(4):234.

  195. Vijaya L, Madhavan HN. In vitro effects of mitomycin C on human Tenon's capsule fibroblasts & correlation with surgical outcome in glaucoma patients. The Indian Journal of Medical Research. 1997 Mar;105:125-30.

  196. Biswas J, Narain S, Roy S, Madhavan HN. Evaluation of lymphocyte proliferation assay to purified protein derivative, enzyme linked immunosorbant assay, and tuberculin hypersensitivity in Eales’ disease. InBasic and Clinical Applications of Vision Science 1997 (pp. 199-201). Springer, Dordrecht.

  197. Rao SK, Madhavan HN, Rao G, Padmanabhan P. Fluconazole in filamentous fungal keratitis. Cornea. 1997 Nov;16(6):700.

    198. 1996
  198. Basti, S. Madhavan, H.N., Bansal, A.K. and Reddy, M.K. Adenoviral Keratitis and Hemorrhagic Cystitis. Cornea. 1996; 15:317-319.

  199. Srinivas K. Rao, Madhavan, H.N., PremaPadmanabhan, G. Sita Lakshmi, Kala Natarajan and Dinesh Gard. Ocular Chlamydial Infections Clinicomicrobiological Correlation. Cornea.1996; 15:62-65.

    200. 1995
  200. Biswas J, Madhavan H.N., Gopal L &Badrinath S S: Intraocular tuberculosis. Retina. 1995; 15:461-468.

  201. Tarun Sharma, Lingam Gopal, Sunil Parikh, Badrinath, S.S., Madhavan, H.N. and B.N. Mukesh. Endophthalmitis caused by Anaerobic bacteria. Indian J. Ophthalmol. 1995; 44 : 191-194,.

  202. Sulochana, K.N., Bhooma, V., Madhavan, H.N. and Ramakrishnan, S. High performance liquid Chromatographic method for simultaneous determination of Ampicillin and Sulbactam in Biological samples. Indian J Pharmacol.l995; 27: l89-192.

  203. Biswas, J., Madhavan, H.N., Gopal, L. and Badrinath, S.S. Clinical Features and Virologic Studies in Viral Retinitis. Indian J. Ophthalmol. 1995; 43: 63-68.

  204. Biswas, J., Madhavan, H.N. and Badrinath, S.S. Ocular lesions in AIDS : A Report of First Two Cases in India. Indian J. Ophthalmol.1995; 43: 69-72.

  205. Madhavan, H.N., Biswas, J. Gopal, L., Sharma, T. Investigations in the virological etiology in acute retinal inflammation. Indian J. Med. Res.1995;101 : 233-237.

  206. Madhavan, H.N., Sridhar, B.R., Vijaya, L. and Neelakantan, A. In-Vitro sensitivity of human Tenon’s capsule fibroblasts to Mitomycin C and its correlation with outcome of glaucoma filtration surgery. Ophthalmic Surgery. l995; 26 :61-67.

    207. 1994
  207. Agarwal, V., Biswas, J., Madhavan, H.N., Mangat, G., Reddy, M.K., Saini, J.S., Savithri, S. and Srinivasan, M. Current perspectives in infectious keratitis. Indian J. Ophthalmol. 1994; 42:171-191.

  208. Madhavan, H.N., Rao, S.K., Kala Natarajan, Sitalakshmi, G., Jayanthi, I. and Sara Roy. Evaluation of laboratory tests for diagnosis of chlamydial infections in conjunctival specimens. Indian J. Med. Res. l994; 100 :5-9.

  209. Madhavan, H.N. and Jayanthi, I. Role of Adenovirus antibodies in decreased infectivity of the virus in conjunctival specimens preserved at -70oC. Indian J. Virol. 1994; 10: 64-66.

    210. 1993
  210. Madhavan, H.N., Jayanthi, D. and Thirunavukkarasu, S. Comparative in-vitro activities of five antibacterial agents on aerobic bacteria isolated from external eye infections. Indian J. Med. Microbiol. 1993; 10: 260-264.

  211. Sithalakshmi, G., Madhavan, H.N., Padmanabhan, P. and Badrinath, S.S. The Efficacy and Safety of Ciprofloxacin Ophthalmic Solution (0.3%) in the Treatment of Corneal Ulcers : A Clinical and bacteriological Study. Asia-Pacific J. Ophthalmol. 1993; 5: 26-29.

    212. 1992
  212. Madhavan, H.N. Detection of Chlamydia trachomatis antigen in McCoy cells inoculated with conjunctival specimens by modified shell vial method using immunoperoxidase stain. Afro-Asian J. Ophthalmol, 1992; 10: 117-119.

1994
Dr. H N Madhavan, received Dr. D.R. Varman Endowment Oration Award for the year l994 by the management of the college of Independent Medical Practitioners of India in recognition of my services in the field of Microbiology and Virology in India.

Dr. H N Madhavan, received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 1994 on his research work.


1995
Dr. H N Madhavan, received Dr. K.C. Basu Mallik Award for the Best Research Work done at the 44th Annual National Conference of Indian Association of Pathologists and Microbiologists held at Patna in 1995.


1996
Dr. H N Madhavan, received The Indian Association of Medical Microbiologists Endowment Award – a Gold Medal and cash award for the year 1996 at XXI National Conference at V.M. Medical College, Sholapur.


1997
Dr. A. R. Anand, received CCLRU Travel Fellowships for attending Indian Eye Research Group Meeting.


1999
Dr. H N Madhavan received Bireswar Chakrabarti Memorial Lecture delivered at the 8th Annual meeting of Indian of Eye Research Group in 1999 for the outstanding contribution in Basic Research in Ophthalmology.

Dr. A. R. Anand, received the CCLRU Travel Fellowships for attending Indian Eye Research Group Meeting.

Dr. J. Malathi, received Dr. H.N. Madhavan Endowment Best outgoing student award in MS-MLT year 1995-1998.

Dr. J. Malathi, received Hi Media Laboratories Pvt. Ltd Endowment Award for best student in Microbiology year 1995-1998.

Dr. J. Malathi, received Best Research Paper Award on 11th October, 1999 at the Centenary celebrations of King Institute and Preventive Medicine, Guindy.

Dr. A. R. Anand, received best Poster Award at XXV National Congress of Indian Association of Medical Microbiologists (IAMM), Chandigarh, India.


2000
Dr. H N Madhavan received Dr. D. Govinda Reddy Memorial Award - Gold Medal and citation for the year 2000 by NTR University of Health Sciences, Vijayawada.

Dr. K. Lily Therese, received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 2000 on her research work.

Dr. A. R. Anand received CCLRU Travel Fellowships for attending Indian Eye Research Group Meeting.

Dr. B. Mahalakshmi received Dr H. N. Madhavan Endowment Award for the Best Student -2000 in MSMLT.

Dr. B. Mahalakshmi received Hi Media Laboratories Pvt Ltd Endowment Award for the best performance in the examinations on Diagnostic Microbiology in MSMLT.

Dr. K. Priya received Junior Best paper Award, XXIV IAMM Best Research Fellow, VRF 2001- 2002


2001
Dr. H N Madhavan, received Arch of Excellence Award –2001 (Medicare) - Gold Medal and Gem of India Award, 2001 by All India Achievers Association.

Dr. A. R. Anand, received Swarnalata Punshi Award (awarded for the best Research Fellow of the year), Medical Research Foundation, Sankara Nethralaya, Chennai, India.


2002
Dr. H N Madhavan, received International Medical Excellence Award & Gold Medal by International Study Circle, 2002.

Dr. K. Priya, received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 2002 on her research work.


2003
Dr. H N Madhavan received Dr. R.V. Rajam Oration award with Gold Medal for the year 2001-2002 by the National Academy of Medical Sciences India at the Annual meeting of National Academy of Medical Sciences (India), the highest body for Medical Academy in India - on 6th September, 2003 at Indore.

Dr. H N Madhavan received Lifetime Service Award for his service to community, our country and indeed to the whole world in the field of Medical Microbiology by the Rotary Club of Chennai Mid City RI District 3230 on 20th December, 2003 at Boat Club, Chennai.

Dr. B. Mahalakshmi received Travel fellowship for the 12th annual meeting of Indian eye research group (IERG), Hyderabad, 2003.


2004
Dr. H N Madhavan received the prestigious Dr. H.I. Jhala award during the annual meeting of Indian Association of Medical Microbiologists at Lucknow.

Ms. P. Deepa received Best Poster Award, Indian Association of Medical Microbiologists at Lucknow.

Dr. B. Mahalakshmi received Junior Best Paper Award 2004 in the XXVIII Annual Congress of Indian Association of Medical Microbiologists at Lucknow.


2005
Ms. G. Shyamala received Best Research Paper in Virology, IJMM – 2005

Dr. R. Bagyalakshmi received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 2005 on her research work.


2006
Dr. K. Lily Therese received Alcon Best Researcher Fellowship Award in Microbiology.


2007
Dr. B. Mahalakshmi received IAMM Silver jubilee award, for best oral presentation in parasitology at the XXXI Annual Congress of Indian Association of Medical Microbiology at Mangalore.

Dr. B. Mahalakshmi received Travel fellowship for the 16th annual meeting of Indian eye research group (IERG), Hyderabad.


2008
Dr. H N Madhavan received the “Genomic Pioneer Award” for Outstanding Contributions in the field of Genomics - Ocimum Biosolutions .The award was announced on 30th Sep ‘08 at HUGO’s International genomic Meeting held at Hyderabad.

Dr. B. Mahalakshmi received Second Prize for oral presentation in International Conference on Nano-Medicine and Recent Advances in Ophthalmic Research, 2008 at Chennai

Dr. P. Sowmya received VRF - IAMM Best paper award, IAMM (TN & Py Chapter) 2008.


2009
Dr. J. Malathi received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 2009 on her research work.

Dr. B. Mahalakshmi received Travel fellowship for the ASIA-ARVO 2009, International meeting on Research in Vision and ophthalmology, 2009, at Hyderabad, India

Dr. R. Gayathri received Second best poster presentation award, National Conference on Emerging trends in life sciences research, BITS Pilani- 2009

Ms M. Sowmya received Best oral presentation award, Asia ARVO.

Ms H. Anusha received VRF - IAMM Best paper award, IAMM (TN & Py Chapter).


2010
Dr. B. Mahalakshmi received International Travel grant- ARVO 2010, International meet on Research in Vision and Ophthalmology, Fort Lauderdale, Florida, USA, May 2nd- May 6th 2010

Ms. L. Dhanurekha received Endowment award for Clinical Genetics instituted by Bangalore Genei Pvt. Ltd

Ms. M. Vimalin Jeyalatha recived Immuno Diagnostic Division Endowment award for Clinical Immunology instituted by Ranbaxy Laboratories Ltd

Ms. A. Shabna received Endowment award for Biochemistry instituted by Wipro biomed Pvt. Ltd

Ms. A. Shabna received Dr. S. Ramaswamy & Dr. S. Narasimhan Endowment award for Human Anatomy & Human Physiology


2011
Dr. H N Madhavan received Institute of Economic studies “International Medical Excellence Award” along with “Certificate Of Medical Excellence The Contribution Towards that Industrial Development at a seminar on 8th October 2009 New Delhi.

Dr. H N Madhavan received Life time achievement award” for the year 2011 by the Executive committee members of Indian Association of Medical Microbiologists (IAMM Conference) during the XXXV IAMM National congress conducted at Banaras Hindu University, Varanasi.

Dr. B. Mahalakshmi received Dr. S.R. Sengupta – Dr. A.M. Saoji Memorial Prize for the Best paper in Immunology 2011, at the XXXV National conference of the IAMM held at Varanasi between 23rd and 26th November 2011.

Ms M. Sowmya received VRF – IAMM Young Best Paper Award TN & PY at Aravind Eye hospital, Madurai

Ms.M.K.Janani received Travel fellowship for the 19th annual meeting of Indian eye research group (IERG), Hyderabad, 2011


2012
Dr. R. Gayathri received Prof. S.Krishnamoorthy Rao Gold Medal Award for the outstanding Ph.D student .

Ms.M.K.Janani received VRF ‐ IAMM Young Microbiologist Best paper award 2012


2013
Dr. K. Lily Therese received recognition of the contribution in “Molecular Microbiology” for Women Alumni of Madras University 2010

Dr. K. Lily Therese received the Best paper in bacteriology entitled “Diagnostic appraisal of simultaneous application of two nested PCRs targeting MPB64 gene and IS6110 region for rapid detection of M. tuberculosis genome in culture proven clinical specimens” published in the Indian Journal of Medical Microbiology for year 2013

Dr.M. Sowmya, received the Prof. S.Krishnamoorthy Rao Gold Medal Award for the year 2013, for the outstanding Ph.D student

Ms. M. Vimalin Jeyalatha, Ms.A.Shabna, Mr. N. Murugan Research Scientists were awarded Travel grant to attend Asia ARVO Conference to be conducted at New Delhi (28-31st October 2013)

Ms. A. Shabna, received the “Swarnalatha Punshi Award” for the best researcher in Basic Medical sciences, for the year 2013 on her research work.

Dr.L.Dhanurekha, received the Young Woman Scientist Award in memory of Smt.Sreerangamma Narayan and Smt.Radhamani N. Anantha for the year 2013


2014
Ms.M.K.Janani received the Young Woman Scientist Award in memory of Smt. Sreerangamma N. Narayan and Smt. Radhamani N. Anantha for the year 2014


2015
Ms.A.R.Rajalakshmy, Ph received the Young Woman Scientist Award in memory of Smt. Sreerangamma N. Narayan and Smt. Radhamani N. Anantha.

Ms. M. K. Janani won the First place in the poster presented at conference held on 28th September 2015 organized by the department of Microbiology in the association with IAMM- Tamilnadu chapter and ICMR.

Ms. Vimalin Jeyalatha won the Second place in the poster presented at conference held on 28th September 2015 organized by the department of Microbiology in the association with IAMM- Tamilnadu chapter and ICMR.

Mr. N. Murugan, Senior Research Fellow from L & T Microbiology Research Centre, won the “Belgaum-Mumbai Best Paper in Bacteriology” award 2015 at the 39th National Conference of the IAMM held at JIPMER, Pondicherry from 27th to 29th November 2015.


2016
Dr. M.K.Janani won the First prize for the oral research paper presentation entitled “Effect of lignocaine concentration on human fibroblasts growth in eyes undergoing trabeculectomy: An in-vitro study” at “World Congress of Ophthalmic Anaesthesia” held on 4th September 2016


2017
Dr.B.Mahalakshmi & Dr.J.Malathi received the best Woman Scientist Awards in memory of Smt. Radhamani N. Anantha & Smt. Sreerangamma N. Narayana endowment awards on 20th September 2017


2018
Dr. K. Lily Therese received “Life Time Achievement Award” at 16th Indian Association Of Applied Microbiologist (IAAM) National Conference at the Department of Water & Health - Faculty of Life Sciences, JSS Academy of Higher Education and Research, Mysuru on 27th September 2018.

Dr.N.Murugan received the best Scientist Award in memory of “Smt. Radhamani N. Anantha ” Dr. Durgadevi received the best Woman Scientist Awards in memory of Smt. Sreerangamma N. Narayana.


MAJOR ACHIEVEMENTS:

1. THE FIRST PATENT CERTIFICATE FOR VISION RESEARCH FOUNDATION

The first Patent certificate for Vision Research Foundation has been granted to the patentee Vision Research Foundation by the Patent office Chennai, Government of India for an invention entitled “A Semi nested Polymerase Chain Reaction (PCR) For Rapid Detection And Specific Identification Of Mycobacterium fortuitum” on 29th May 2009 for the term of 20 years from the 12 day of July 2005 in accordance with the provisions of the Patents Act 1970 (The Patent No is 232658 934/ CHE/ 2005). The inventors are Dr. K. Lily Therese, Ms. Jasmine Therese and Dr. H.N. Madhavan. This is the first patent received by scientists in Vision Research Foundation.


2. THE FIRST PATENT CERTIFICATE FOR VISION RESEARCH FOUNDATION


Vision Research Foundation has been granted to the patentee Vision Research Foundation by the Patent office Chennai, Government of India for an invention entitled “A Semi nested Polymerase Chain Reaction (PCR) Method For Rapid Detection And Specific Identification Of Mycobacterium Chelonae” on 12th November 2009 for the term of 20 years from the 13 day of July 2005 in accordance with the provisions of the Patents Act 1970 (The Patent No is 236495, 937/ CHE/ 2005). The inventors are Dr. K. Lily Therese, Ms. Jasmine Therese and Dr. H.N. Madhavan.


3. THE THIRD PATENT CERTIFICATE FOR VISION RESEARCH FOUNDATION


THE THIRD Patent Certificate for Vision Research Foundation has been granted to the patentee Vision Research Foundation by the Patent office Chennai, Government of India for an invention entitled “A method for Cultivating Cells derived from Corneal Limbal Tissue and Cultivation method using Mebiol gel (Thermoreversible polymer)” provided by Professor Y. Mori of Waseda University Tokyo, Japan” on 14th June 2010 for the term of 20 years from the 28 day of March 2005 in accordance with the provisions of the Patents Act 1970 (The Patent No is 239350, 4226/CHENP/2007) Dr. H. N. Madhavan is the inventor of this patent


4. THE FOURTH PATENT CERTIFICATE FOR VISION RESEARCH FOUNDATION

THE FOURTH Patent Certificate for Vision Research Foundation has been granted to the patentee Vision Research Foundation by the Patent office Chennai, Government of India for an invention entitled “A multiplex Polymerase chain reaction for rapid detection and specific identification of Cytomegalovirus” in accordance with the provisions of the Patents Act 1970 (The Patent No is) Dr. H. N. Madhavan Dr.P.Sowmiya and DR.K.Lily Therese are the inventors of this patent



V PATENT

“A Novel Method For Simultaneous Detection And Discrimination Of Bacterial, Fungal, Parasitic And Viral Infections Of Eye And Central Nervous System”, Macro DNA chip for detection of 14 ocular infectious agents



MACRO DNA VISION CHIP” FOR AETIOLOGICAL DIAGNOSIS OF OCULAR INFECTIONS


The team led by Prof. H N Madhavan from L & T Microbiology Research Centre, Vision Research Foundation worked for 4 ½ years on the project with a grant of Rs 3.5 crores under the New Millennium Indian Technology Leadership Initiative (CSIR- NMITLI) programme on Ocular Infectious Diseases group for a Novel molecular diagnostics

“Xcyto-screen DNA chip” is a diagnostic product of collaborative research of four Ophthalmic centres viz. Vision Research Foundation, Chennai; L.V. Prasad Eye institute, Hyderabad; Dr. Rajendra Prasad Centre for Ophthalmic Sciences, AIIMS, New Delhi; Centre for Cell and Molecular biology, Hyderabad and Industrial partner, Xcyton Diagnostics Pvt., Ltd., Bangalore. The DNA chip aids in the diagnosis of the following four ocular clinical conditions:

Clinical validation of the X cyto-Screen kits was carried out by the team at L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai. The product was launched on 22nd September 2007 in Bangalore.

The total time taken for the test to be performed is eight hours. The cost per test is Rs. 2, 500/-. The validation of the DNA chip was carried out under personal supervision of Dr. H. N. Madhavan at L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, 18, College Road, Chennai – 600-006


The advantages of the vision chip are

  • A Single chip can detect probable infectious etiology of a given clinical condition in a Multiplex format.

  • A significant reduction in cost, time and labour.

  • Reduced risk of cross-contaminations as nested round is avoided.

  • Sensitivity is equivalent to individual nested PCRs by an improved detection system

  • No carcinogenic chemicals are used in the test.

  • Detection of the amplified product does not require ultraviolet radiation or Radiographic or Spectrophotometric methods.

The DETECTION IS DONE BY NAKED EYE

Though this is Polymerase Chain Reaction-based technique, a trained medical laboratory technician can perform the test with ease. Even laboratories at the district level hospital can introduce this test.



DR. S.S. BADRINATH, CHAIRMAN EMERITUS, SANKARA NETHRALAYA RECEIVING THE FIRST VISION CHIP KIT FROM T. RAMASAMI, SECRETARY, DST DURING THE LAUNCH OF THE KIT


Dr. H.N. MADHAVAN, DIRECTOR OF RESEARCH, SANKARA NETHRALAYA FELICITATED BY DR. T. RAMASAMI, SECRETARY, DST

The kit was launched on Saturday 22nd September 2007 by our beloved Chairman Emeritus, Sankara Nethralaya, Dr. S. S. Badrinath and Dr. T. Ramasami, Secretary, Department of Science and Technology. The kit was officially launched on behalf of CSIR, the Research Scientists at Vision Research Foundation, Sankara Nethralaya, Chennai; LVPEI, Hyderabad; RP centre for Ophthalmology, AIIMS, New Delhi and Centre for Cellular and Molecular Biology, Hyderabad and X Cyton Diagnostics Ltd, Bangalore (the Industrial partner) at Taj Gateway Residency in Bangalore. Dr. G. Padmanabhan of Indian Institute of Science presided over the function.


VI FIRST PRIVATE LABORATORY IN CHENNAITO GET RNTCP RECOGNITION BY CENTRAL TB DIVISION MINISTRY OF HEALTH, GOVERNMENT OF INDIA & WHO FOR ISOLATION AND DST FOR IST LINE DRUGS OF M. TUBERCULOSIS


CONFERENCE / PCR WET WORKSHOP CONDUCTED BY THE CENTRE – UNDER THE AEGIS OF IAMM

  • Conducted One day Chapter meet of IAMM (Indian Association of Medical Microbiologists) – Tamil Nadu and Pondicherry Chapter on 24th January 1997 in which more than 75 delegates participated.

  • PCR Wet Workshop on “PCR for detection of infective agents in clinical specimens” on 19th and 20th April 2002. There were 25 participants (Assistant / Associate Professors and Post Graduates) from Medical Colleges and Research Institutions from Tamil Nadu and Pondicherry.


  • One day Chapter meet of Indian Association of Medical Microbiologists – TamilNadu and Pondicherry Chapter on 23rd August 2003, on the theme “ Application of RT - PCR and Microarray in Microbiology” in which there were more than 150 delegates from TamilNadu and Pondicherry

  • PCR Wet Workshop on “PCR for detection of infectious agents” on 19 and 20 October 2005. There were 25 participants (Assistant / Associate Professors and Post Graduates) from 14 Medical Colleges and Research Institutions from all over the country. It was well appreciated by the participants.


  • On 24th February 2007, we conducted a one-day CME programme jointly with Indian Association of Medical Microbiologists (IAMM) There were 250 participants including Research scientists from Microbiology department, Sankara Nethralaya , There were two guest lectures on the theme of the CME ”Newer antituberculosis drugs: antibiotics from untapped sources and from synthesized compounds“ and 17 paper presentations by Post Graduates / Research scientists from 7 Institutions in and around Tamil Nadu and Pondicherry region.

  • The three day Wet Workshop on “Recent techniques in Molecular Microbiology” between 11, 12 &13th February 2010 for IAMM members under the banner of VRF-IAMM TN Chapter. Dr. K. Lily Therese – Convenor, Dr. J. Malathi is the Organising Secretary & Dr. B. Mahalakshmi – Treasurer. There were 21 participants. The Participants were faculty from 9 Medical colleges from Tamil Nadu and one from Kerala. The participants were given hands-on-training on Polymerase Chain Reaction (PCR), Reverse-transcriptase PCR (RT- PCR). There was demonstration on Real time PCR, DNA sequencing & PCR-RFLP techniques.

  • The WHONET training Programme Conducted under VidyaSagar Institute of Biomedical Technology and Science at L & T Microbiology Research Centre from 21st -25th March 2011 on “Bi-regional Workshop on Laboratory Based Surveillance of Antimicrobial Resistance”. There were 18 (17-International and 1- National) participants and 6 Facilitators (4 from WHO, 2 from SN).


  • The WHONET training Programme Conducted under VidyaSagar Institute of Biomedical Technology and Science at L & T Microbiology Research Centre from17th June to 21st June 2013, “Bi-regional Workshop on Laboratory Based Surveillance of Antimicrobial Resistance”. 22 Microbiologists from south East Asia region participated in this training program.


    • INSTITUTION OF VRF IAMM BEST PAPER AWARD

  • L & T Microbiology Research Centre, Vision Research Foundation instituted an award in the name of “VRF-IAMM Tamil Nadu & Pondicherry chapter” for best paper award to a young scientist with a cash prize and a certificate to be presented to the winner every year during one of the IAMM local chapter meeting.

  • In the year 2008, Ms. P. Sowmya Senior Research Fellow, L & T Microbiology Research centre received the first award of VRF-IAMM Best paper award for her presentation of a research paper entitled “Analysis of mixed infections with multiple genotypes of Human Cytomegalovirus in immunocompromised patients” during the SOZOCON– MICRO 2008 jointly organized by IAMM (TN & Py chapter) at Sri Manakula Vinayagar Medical college and Hospital, Puducherry on 19th January 2008

  • In the year 2009, Ms. H. Anusha Senior Research fellow, L & T Microbiology Research centre received the first award of VRF- IAMM Best paper award for her presentation of a research paper entitled “Phylogenetic analysis of clinical Herpes Simplex Virus 1 isolates targeting 269 nucleotide region within glycoprotein G and 410 nucleotide region within glycoprotein I” in TN IAMM local chapter meeting on Hospital acquired infection at Madurai Medical College, Madurai (HAICON 2009) on 2nd May 2009.

  • In the Year 2010, Dr. K. Subha, Stanley Medical college Hospital, received the award of VRF- IAMM Best paper award for her presentation of a research paper entitled “Bacteriology of abscesses with special reference to phenotyping and genotyping of Methicillin resistant Staphylococcus aureus in Stanley medical college hospital, Chennai, Tamilnadu” during the CME Programme conducted under the banner of IAMM at Kanyakumari Medical College at Nagercoil on 29th July 2010.

  • In the year 2011, Ms. M. Sowmiya, Junior Scientist, received the award for her research paper entitled “Prevalence of Extended Spectrum Beta-Lactamase (ESBL) producing and integrons carrying Gram negative bacilli belonging to Enterobacteriaceae among Ocular isolates - A hospital based study” during the CME programme on “Ocular infections 2011” conducted on 23rd July 2011 organised by Department of Microbiology, Aravind Eye Hospital, Madurai as part of IAMM - TN & Pondicherry chapter meet.

    • JOINT VENTURE OF EXTERNAL QUALITY CONTROL ASSESSMENT SCHEME (EQAS) UNDER THE AEGIS OF IAMM

  • As decided at the Annual Congress in the General Body Meeting of Indian Association of Medical Microbiologists (IAMM) 2006 in Nagpur, External Quality Assessment System programme (EQAS) was conducted as a joint venture by L & T Microbiology Research Centre, Sankara Nethralaya and Amala Medical institute, Thrissur, Kerala from January 2007 - 2016.

  • The programme involved distribution of four Quality control packages sent during the months of January, April, July and October, every year. Each package consists of a 1) Direct Smear for evaluation, 2) Culture for identification of a bacteria/ fungal pathogen isolated from clinical specimens and serological testing to be carried out by the participant.There were 600 members belonging to Medical colleges, Medical Institutions and Private clinical laboratories as participants in the programme.

  • Participation in EQAS enables clinical laboratories to assess their status regarding the quality of techniques followed, skill of the laboratory staff. The participation in EQAS programme also is essential for getting National Accreditation Board for Laboratories certification (NABL).


L & T MICROBIOLOGY RESEARCH CENTRE


PH.D AWARDEES FROM L & T MICROBIOLOGY RESEARCH CENTER

S.No Name Title University
1 Dr A R Anand- 2001 “Evaluation of Molecular techniques in the diagnosis of infectious endophthalmitis” TN Dr. M. G. R Medical University, Chennai
2 Dr K Priya- 2002 “The role of Molecular biological techniques on Clinical specimens in Human Infections due to Herpes Simplex Virus(HSV), Varicella Zoster Virus (VZV) and Cytomegalovirus (CMV)” Birla Institute of Technology and Science(BITS,)Pilani
3 Dr J Malathi- 2003 “Molecular methods in the characterization of Adenoviruses and Chlamydia trachomatis detected in Clinical specimens” BITS, Pilani
4 Dr R Bagyalakshmi - 2008 “Role of nucleic acid based amplification technique in detection, characterization and species level identification of fungi in clinical specimens and a study of in vitro sensitivity pattern of ocular fungal isolates” BITS, Pilani
5 Dr G Shyamala - 2008 “Nucleic Acid based Amplification methods for the detection, Quantification and Characterization of Viruses in Clinical Specimen” BITS, Pilani
6 Dr B Mahalakshmi - 2008 “Role of Nucleic acid based–molecular biological techniques and evaluation of serological methods in the laboratory diagnosis of ocular toxoplasmosis and genotyping of Toxoplasma gondii ” BITS, Pilani
7 Dr P Deepa - 2008 “Role of Nucleic Acid Based Amplification tests in the Detection & Identification of Mycobacterial Infections and Determination of Sequence Variation & Rifampicin Resistance Among Mycobacterium tuberculosis Isolates “ TN. Dr. M. G. R Medical University, Chennai
8 Dr P Sowmya - 2009 “Nucleic acid based molecular biological methods for quantization and characterization of Human Cytomegalovirus in the clinical specimens from immunocompromised patients” TN. Dr. M. G. R Medical University, Chennai
9 Dr R Gayathri-2012 Rapid detection of M. tuberculosis by molecular methods from clinical specimens and genotypic drug susceptibility testing of M. tuberculosis BITS, Pilani
10 Dr M Sowmiya-2012 Detection of drug resistance exhibited by Gram-negative bacteria isolated from clinical specimens to quinolones and ESBL group of drugs by molecular methods. BITS, Pilani
11 Dr Y Samson Moses-2012 Application of Molecular methods in detection of mutation in UL23 gene of HSV and UL97 gene of Human Cytomegalovirus for drug resistance and evaluation of pp65 antigenemiaagainst Real time PCR assay in monitoring drug therapy in HCMV infections. BITS, Pilani
12 Dr L Dhanurekha-2015 Phenotypic drug susceptibility testing and detection of resistance by PCR based DNA sequencing to first and second line drug in M. tuberculosis isolates circulating in Chennai population. BITS, Pilani
13 Dr M K Janani-2016 A Clinico - Molecular Biological and Genotypic Study on Epstein - Barr virus in Chennai Population. BITS, Pilani
14 Dr.A.R.Rajalakshmy-2016 Molecular Immunology of Ocular Manifestations and Neuroinvasion of Hepatitis C Virus: Immune Response Against Virus and Viral Antigens. SASTRA University Thanjavur
15 Dr M Vimalin Jeyalatha-2016 Prevention of Corneal Limbal Epithelial Cell Death during Collagen Crosslinking and the Pathogenic Mechanisms Involved in Progression of Keratoconus BITS, Pilani
16 Dr. A.Shabna-2016 In vitro evaluation of the scope of an indigenously produced Poly hydroxyalkanoate based co-polymer developed from Pseudomonas MNNG-S in cell culture and virology BITS, Pilani
17 Dr Murugan-2017 NGS based Study on Genome, Drug resistance, Virulence Mechanism of Multi Drug Resistant Pseudomonas aeruginosa and its molecular diagnosis toIn silico drug discovery SASTRA University Thanjavur

TRAINING PROGRAMMES CONDUCTED IN L&T MICROBIOLOGY RESEARCH CENTRE UNDER VIDYASAGAR INSTITUTE OF BIOMEDICAL TECHNOLOGY AND SCIENCE (VIBS)

Dr. AR.ANAND- Programme Co-ordinator -VIBS

Padma shri. Dr. Irvatham Mahadeven- Established Vidya Sagar Institute of Biomedical Technology and Science (VIBS), a unit of Medical Research Foundation in memory of his elder son, late shri Vidyasagar, on 25th October 2006.

In the year 2006 short term (3-4 months from December to next year March) research project for final year post graduate students from Arts and Science College / Engineering college was initiated under the aegis of Vidya Sagar Institute of Biomedical Technology and Science (VIBS).


L&T MICROBIOLOGY RESEARCH CENTRE
SUMMER TRAINING PROGRAMME

One month hands-on training programme for postgraduate students in biological sciences studying in science colleges during May – June (summer holidays) was started in the year 2003 in Microbiology department on several biomedical technologies such as nucleic acid based molecular biological techniques like Polymerase Chain Reaction for detection of infectious agents, ELISA, tissue culture and virology under the aegis of Vidya Sagar Institute of Biomedical Technology and Science (VIBS).


SHORT TERM RESEARCH PROJECTS

Training programme Year Total Number of
students completed
BITS PS (I) Summer school projects 1997 - 2015 45
Hands on summer training programme 2003- 2021 66
3-4 months Internship projects 2005 - 2021 68

OTHER TRAINING & OBSERVERSHIP PROGRAMMES FROM L & T
MICROBIOLOGY RESEARCH CENTRE (2006 to 2018)

S.No Year Number of Students
1 2006 - 2007 7
2 2007- 2008 4
3 2008 - 2009 3
4 2009 - 2010 6
5 2010 - 2011 6 (3 National & 3 International)
6 2011 - 2012 4 (1 National & 3 International)
7 2012 - 2013 5 (2 National & 3 International)
8 2013 - 2014 2
9 2014 - 2015 4
10 2015 - 2016 1
11 2016 - 2017 5
12 2017 - 2018 5
13 2018 - 2019 7
14 2019 - 2020 7
Total 66



List of equipments in L & T Microbiology Research Centre Vision Research Foundation

S.No Equipment Name Nos
1 Anaerobic Work Station 1
2 Autoclaves 2
3 Binocular microscopes 4
4 Bio Safety Cabinet Type I 2
5 Bio Safety Cabinet Type II 3
6 Bio Safety Cabinet Type III 2
7 Carbon-di-oxide (CO2 ) incubators 3
8 Centrifuges 6
9 Cooling Centrifuges 2
10 Cyclomixer 6
11 Cytospin instrument 1
12 Deep Freezer - 20°C 3
13 Deep Freezer - 80°C 2
14 Denaturing high performance liquid chromatography (dHPLC) 1
15 Electronic weighing balance 2
16 Electrophoresis apparatus 4
17 ELISA reader (Biotek instruments inc) 2
18 ELISA Washer (Biotek instruments inc) 2
19 Eppendorf Plate Centrifuge 1
20 Fluorescence Microscope 2
21 Foot wear Machine 1
22 Gel documentation system 1
23 Hot air oven 1
24 Hot Plate 1
25 Inverted phase contrast microscope 2
26 Incubator (Aerobic) : 42°C 1
27 Incubator (Aerobic) : 37°C 6
28 Incubator (Aerobic) : 25°C 1
29 Ion Torrent Personal genomics machine 1
30 Laminar Flow Hoods – Type I 4
31 Lyophilizer 2
32 Magnetic Stirrer 1
33 Microfuges 3
34 Microwave oven 1
35 PCR machines 6
36 PAGE Apparatus 1
37 Real time PCR machine 1
38 Refrigerators 6
39 Rockers 3
40 Speed vac concentrator 1
41 Spinwin 1
42 UltraSonicator 1
43 Vaccum Pump 1
44 VDRL Rotator 1
45 Water baths 3
46 Vitek 2 automated ID System 1
Larsen & Toubro Microbiology Research Centre and SAIL Molecular Microbiology Centre
Vision Research Foundation,
18, College Road, Nungambakkam,
Chennai - 600 006.
Phone: +91-44-42271807, +91-44-42271500, (Extn: 1301)
Email: microbiology@snmail.org