RESEARCH EXPERIENCE

Completed Projects with title and source of funding

S.No	Title of Project	Role	Funding Agency
1	Aptamer toxin conjugates for targeting cancer (stem) cells	PI	DBT, GOI
2	Fabrication of PDMS based micro PCR for the detection of oncogenic miRNAs in retinoblastoma patients serum	PI	NPMASS-ADA-DRDO
3	To Engineer a Aptamer-siRNA chimeric conjugates for targeting epithelial cancers	Co-I	DBT, GOI
4	International workshop on MEMS and its health care applications (Indo-Australia grant)	Co-PI	DBT-AISRF
5	Self-assembling peptide nanofiber matrix for drug delivery to fungal keratitis	PI	DBT
6	Mechanobiological effect of chemotherapeutic drugs on retinoblastoma	PI	ICMR

Google Scholar : Check out this link

List of publications

1. Jayavigneeswari Suresh babu, Aravindan Saravanan, Bharathselvi Muthuvel, Ronnie George, Janakiraman Narayanan, Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems, OpenNano,2023, 100122, ISSN 2352-9520, https://doi.org/10.1016/j.onano.2023.100122


2. Gopal, D., Nagarajan, H., Muthuvel, B., Vetrivel, U., George, R., & Janakiraman, N. (2022). Synthesis and Characterization of a Novel Peptide Targeting Human Tenon Fibroblast Cells To Modulate Fibrosis: An Integrated Empirical Approach, ACS Pharmacology & Translational Science, 5(12), 1254-1266, https://doi.org/10.1021/acsptsci.2c00148


3. Gopal Divya, Ramani Madhura, Vikas Khetan, Pukhraj Rishi, Janakiraman Narayanan, Understanding the mechano and chemo response of retinoblastoma tumor cells: Mechanobiology of retinoblastoma, OpenNano, 2022, 100092, ISSN 2352-9520, https://doi.org/10.1016/j.onano.2022.100092.


4. Amit, C., Ghose, V., Narayanan, J., Padmanabhan, P., Sathe, G., & Elchuri, S. (2022). Phosphoprotein network analysis of corneal epithelium of Keratoconus patients. Proteomics, 2100416. https://doi.org/10.1002/pmic.202100416


5. Chatterjee, A., Ansar, S., Gopal, D., Vetrivel, U., George, R., & Narayanan, J. (2022). Elucidating the Therapeutic Potential of Cell-Penetrating Peptides in Human Tenon Fibroblast Cells. ACS Omega. https://doi.org/10.1021/acsomega.2c00701


6. Gopal, D., Ramani, M., George, R., & Janakiraman, N. (2022). Understanding the cellular response of human tenon fibroblast on polycaprolactone-Aloe vera blend fiber. Journal of biomaterials applications, 8853282221091042. Advance online publication. https://doi.org/10.1177/08853282221091042


7. Nakagawa, Tatsuya, Naoki Okumura, Naoya Hanada, Prema Padmanabhan, Sailaja Elchuri, Amit Chatterjee, Narayanan Janakiraman et al. "Proteomic analysis of TCF4 knock-out corneal endothelial cells derived from the patients with Fuchs endothelial corneal dystrophy." Investigative Ophthalmology & Visual Science 62, no. 8 (2021): 827-827.


8. Amit, C., Sathe, G., Shunmugam, A., Athyala, P. K., Ghose, V., Chitipothu, S., Janakiraman, N., Sundara, R., & Elchuri, S. V. (2021). Graphitic Carbon Nitride Causes Widespread Global Molecular Changes in Epithelial and Fibroblast Cells. ACS omega, 6(14), 9368–9380. https://doi.org/10.1021/acsomega.0c05513


9. Sivagurunathan, S., Selvan, L., Khan, A. A., Parameswaran, S., Bhattacharjee, H., Gogoi, K., Gowda, H., Keshava Prasad, T. S., Pandey, A., Kumar, S. A., Rishi, P., Rishi, E., Ratra, D., Bhende, M., Janakiraman, N., Biswas, J., & Krishnakumar, S. (2021). Proteomics-based approach for differentiation of age-related macular degeneration sub-types. Indian journal of ophthalmology, 69(3), 647–654. https://doi.org/10.4103/ijo.IJO_470_20


10. Amit, C., Padmanabhan, P., & Narayanan, J. (2020). Deciphering the mechanoresponsive role of β-catenin in keratoconus epithelium. Scientific reports, 10(1), 21382. https://doi.org/10.1038/s41598-020-77138-3


11. Exosome: A Model to Study the Protein-Protein Interaction in Biofluids JN Amit Chatterjee, EC Ophthalmology 12 (5), 5-15


12. Agarwal, S., Srinivasan, B., Janakiraman, N., Therese, L. K., S, K., Patel, N., V, T., & Iyer, G. (2020). Role of Topical Ethanol in the Treatment of Pythium insidiosum Keratitis-A Proof of Concept. Cornea, 39(9), 1102–1107. https://doi.org/10.1097/ICO.0000000000002370


13. Chatterjee, A., Nagarajan, H., Padmanabhan, P., Vetrivel, U., Therese, K. L., & Janakiraman, N. (2020). Understanding the Uptake Mechanism and Interaction Potential of the Designed Peptide and Preparation of Composite Fiber Matrix for Fungal Keratitis. ACS omega, 5(21), 12090–12102.https://doi.org/10.1021/acsomega.0c00321


14. Amit, C., Padmanabhan, P., Elchuri, S. V., & Narayanan, J. (2020). Probing the effect of matrix stiffness in endocytic signalling pathway of corneal epithelium. Biochemical and biophysical research communications, 525(2), 280–285.https://doi.org/10.1016/j.bbrc.2020.02.067


15. Chatterjee A, Muralikumar S, Janaki S, Lakshmipathy M, Therese KL, Umashankar V, Padmanabhan P, Narayanan J. Design and enhancing the anti-fungal activity of corneal epithelial specific cell penetrating peptide using gelatin hydrogel delivery system. International Journal of Nanomedicine. In press 2018


16. Athyala, Prasanna Kumar; Kanwar, Jagat; Chitipothu, Srujana; Subramanian, Krishnakumar, Janakiraman, Narayanan 'Synthesis of Saporin - antibody conjugates for targeting EpCAM positive tumor cells', IET Nanobiotechnology, 2018, DOI: 10.1049/iet-nbt.2018.5222, IET Digital Library.


17. Athyala, Prasanna Kumar, Jagat Rakesh Kanwar, Srujana Chitipothu, Rupinder Kaur Kanwar, Subramanian Krishnakumar, Jonathan P. Watson, and Janakiraman Narayanan. "Neocarzinostatin, Aptamer Conjugates for Targeting EpCAM-positive Tumor Cells." Anticancer research 37, no. 7 (2017): 3615-3629.


18. Athyala, P. K., Kanwar, J. R., Alameen, M., Kanwar, R. K., Krishnakumar, S., Watson, J., ... & Narayanan, J. (2016). Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer. Biochemical and biophysical research communications, 469(2), 257-262.


19. Jayashree, B., Srimany, A., Jayaraman, S., Bhutra, A., Janakiraman, N., Chitipothu, S., ... & Pradeep, T. (2016). Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS. Analytical and Bioanalytical Chemistry, 1-10.


20. Salim, B., Merlin David, S., Beta, M., Narayanan, J., & Krishnakumar, S. (2015). PMMA Platform Based Micro Fluidic Mixer for the Detection of MicroRNA-18a from Retinoblastoma Serum. J Anal Bioanal Tech, 6(258), 2.


21. Subramanian, Nithya, Jagat R Kanwar, Prasanna kumar Athalya, Narayanan Janakiraman, Vikas Khetan, Rupinder K Kanwar, Sailaja Eluchuri, and Subramanian Krishnakumar. 2015. "EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex." Journal of biomedical science (1):4


22. Madhu Beta, Subramanian Krishnakumar, Sailaja V Elchuri,Bindu Salim, Janakiraman Narayanan. 2015. "A Comparative Fluorescent Beacon-based Method for Serum microRNA Quantification." Analytical Sciences 31 (3):231-235.


23. Janakiraman, N., Mohan, A., Kannan, A., & Pennathur, G. (2012). Resonance Energy Transfer between protein and rhamnolipid capped ZnS quantum dots: Application in in-gel staining of proteins. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 95, 478-482.


24. Narayanan, J., R. Ramji, H. Sahu, and P. Gautam. "Synthesis, stabilisation and characterisation of rhamnolipid-capped ZnS nanoparticles in aqueous medium." IET nanobiotechnology 4, no. 2 (2010): 29-34.

Patents filed

1. A Method of isolating synthesizing and characterising peptide sequences for targeting corneal epithelial cells and novel peptide sequences” Application submitted to India Patent Office, Application no. No. 201841043278 dated 16/11/2018


2. Process for preparing rhamnolipid capped metal nanoparticles. PennathurGautam, Narayanan Janakiraman. Centre for Biotechnology, Anna University, Chennai – 600025, Tamil Nadu Patent Application No.2143/CHE/2008. Date of publication: 21/11/2008. The Patent Office Journal


3. A molecular beacon probe and a method for quantification of miRNA copy number directly from biological fluid with molecular beacon probe. Draft filed for Indian Patent office No.3035/CHE/2015 Dated 17/06/2015

List of Invited Talks

1. Dr.J.Narayanan gave invited talk titled “Toxin conjugates for targeting epithelial tumors” at one day conference Recent advances in Nanoscience and technology at Anna University on 7th December 2018


2. Dr.J.Narayanan gave invited talk “Recent advances in Mechanobiology: Regenerative medicine approach” at IERG conference held at LV Prasad Eye Institute, Hyderabad on 27th July 2018


3. Dr.J.Narayanan gave a invited talk titled “Recent advances in mechanobiology: Avenues for targeting Retinoblastoma cancer stem cells” on 5 th December 2017 at Sri Venkateshwara College of Engineering, Chennai in DST sponsored faculty development program (FDP)


4. Dr.J.Narayanan gave a invited talk titled Dual nature of Neocarzinostatin conjugates in targeted tumor therapy“ SERB Sponsored National Conference on NANOTECHNOLOGY IN MEDICINE”8-9th September, 2017 held at Chettinaud Health City


5. Dr.J.Narayanan gave a invited talk titled “Extracellular matrix and cell interaction: Mechanobiological view” at Synergy”– a symposium is being organized under the aegis of the Indo-US collaborative partnership that SN has with Indiana University held between 24 and 25 March 2018


6. Gave a talk titled “ An overview of research activities on MEMS in India” in Collaborative Workshop on Microelectromechanical Systems (MEMS) and their Applications in Health Care” held at Deakin University, Australia: between 10th March-15th March 2014 funded by DBT Indo-Australia Strategic Research Fund


7. Dr.J.Narayanan gave a talk “Application of FRET in detecting biomolecules “ at Deakin University, Australia on 12th March 2014


8. Organised and gave a talk in Clinical Nanomedicine Program between 2-3 August 2013, @ Sri V.D Swami Auditorium conducted by VRF and SN Academy


9. Gave a talk “Bionanosensor for protein/DNA molecules using a FRET (Fluorescence resonance energy transfer)phenomenon at IEEE International conference on Smartstructures and systems” between March 28-29, 2013 at Saveetha Engineering College Chennai.

List of Short Term students completed their post graduate Thesis

1. Uma Maheshwari- B Tech Biotech, SRM University, Chennai


2. Mr.Yogananth- MSc, Biotechnology, GR Domodharan college of Science, Coimbatore


3. Mr.Sivaramakrishnan, M Tech Nanotech SRM University, Chennai


4. Ms.S.Sruthi, IV Year, B.Tech., (Biotechnology), Rajalakshmi Engineering College, Chennai


5. Mr.Shivaramakrishnan IV Year, B.Tech.SathyabamaUniversity,Chennai


6. Mr.Muthukrishna-IV Year B.Tech Biotechnology, Madha Engineering, Kundrathur, Chennai


7. Ms.Swapna-Btech-II Year, M.Sc. Biotechnology,Alpha Arts and Science College, Chennai


8. Ms.KODI SAMEERA-B.Tech Biological Sciences & Electrical & Electronics Engineering, BITS Pilani


9. R. Radha IV Year B.Tech. Biotechnology, St.Joseph College of Engineering,


10. Ms.Sumo Ashok, IV Year B.Tech, Biotechnology student from Sri Venkateswara College of Engineering


11. Ms.Anila Rao KP IV Year B.Tech, Biotechnology student from Sri Venkateswara College of Engineering


12. Prasanna Kumar A II Year, M.Tech.Pharmaceutical Technology, student from Sastra University

List of workshop/seminars/conferences attended in the relevant/associated field

1. Amit Chatterjee, PremaPadmanabhan, Janairaman Narayanan, “Probing the mechanism how matrix stiffness alters the cytoskeletal rearrangement in corneal epithelial cells and regulates the peptide uptake” at EMBO workshop at IISER, Pune, India


2. Janakiraman Narayanan, Kong Fong, CT Lim, “Heterogeneity of elastic modulus in metastatic and non metstatic retinoblastoma (rb) cells and its correlation with cluster size, Mechanobiology of the diseases, Biophysical Society, Singapore, September 27-30, 2016


3. Amit Chatterjee, Janakiraman Narayanan, Subbulakshmi Chidambaram “Extracellular vesicle mediated transport of PIWI like proteins from retinal pigmented epithelial cells to vitreous” Indian Eye Research Group (IERG)-ARVO India chapter, July 30-31, 2016 (Won Travel Grant for the Poster)


4. SivapriyaSivagurunathan,, Krishnakumar S, HarshaGowda, Keshavaprasad, TS, Afaque A Khan, AkileshPandy, Narayana J, Ashok Kumar S “Study on identification of biomarkers for age related macular degeneration “2nd International conference on Biotechnology and Pharmaceutical Sciences, Hyderabad, October 3-4, 2015 (Best Poster Award)


5. Prasannakumar A, JagatKanwar, Jon Watson, Subramanian Krishnakumar, Narayanan Janakiraman “Antibody conjugates for EpCAM positive tumor cells” presented in 7th Bangalore INDIA NANO 2014


6. SukanyaMadan Mohan, Nithya Subramanian, Raghu Sripada, Narayanan Janakiraman, SundaraRamprabhu, Subramanian Krishnakumar, Sailaja V Elchuri “Spectroscopic Characterization of G Quadraplexs in microRNA promoter region” presented at RSC Royal Society of Chemistry symposium held at IIT Madras on 10th Nov 2014. (Best Poster Award)


7. MadhuBetha, Krishnakumar Subramanian, Narayanan Janakiraman “Towards development of possible nanosensor for miRNA using LNA modified Molecular Beacon Probe from the serum” presented in 6th Bangalore INDIA NANO 2013


8. Prasannakumar A, Subramanian Krishnakumar, Narayanan Janakiraman, “Conjugation of aptamer-toxin for targeting tumour cells” presented in 6th Bangalore INDIA NANO 2013


9. GautamParthasarathy, BinduSalim, Krishnakumar Subramanian, Narayanan Janakiraman “Simulation and Design of a Microfludic Device Towards Detection of Tumor Markers in the Serum of Retinoblastoma Patients” Fifth ISSS National Conference on MEMS, Smart Materials, Structures and Systems, September 21-22, 2012, Karpagam University, Coimbatore


10. Study on rhamnolipid capped nanoparticle synthesis in aqueoous medium and its application J.Narayanan, Ramesh Ramji, Hrushikeshsahu and GautamPennathur.The 5th SBE International Conference on Bioengineering and Nanotechnology (ICBN2010), Singapore. 2010.


11. Synthesis and characterization of rhamnolipid capped CdS nanoparticles. Narayanan Janakiraman, K. Sujatha, Ramesh Ramji, K. Kumar and GautamPennathur. ICONSAT-08. Chennai 2008


12. Studies on development of a possible biosensor for quorum sensing in Pseudomonas aeruginosa. Narayanan Janakiraman, AshwinKannan and GautamPennathur. The 8th International Workshop on biosensors and bioanalytical techniques in environmental and clinical analysis. BITS, Goa 2007


13. Primary Microcephaly in Indian families: Genetic and Biochemical aspects Arun Kumar, Narayanan Janakiraman, Satish Chandra Girimaji. International Symposium on Human Genomics and Public Health. New Delhi 2006

2016
ICMR-DHR, Govt of India,long term international fellowship in Mechanobiology at Mechanobiology Institute (MBI) National University of Singapore (NUS), Singapore with Prof. C T Lim between April 2016 to September 2016

Under graduate/Graduate/Post graduate Thesis: If anyone is interested, kindly send me a small write up on research interest and how training will benefit the career. Any short term project will be undertaken through vibs@snmail.org

PhD/MS: Applicants need to have through one of NET/UGC/GATE/INSPIRE/DBT/ICMR fellowships. If anyone is interested can send their CV with one page write up. Depends on the funding availability, decision will be communicated.

Interns: Candidates can send their CV and research interest through Vidyasagar Institute of Bio Medical Sciences vibs@snmail.org

Dr Janakiraman Narayanan, PhD
Department of Nano biotechnology
Vision Research Foundation,
41/18 College Road, Nungambakkam
Chennai – 600 006.
Ph: +91-(044) 28271616 Extn:1358
Email: drnj@snmail.org