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Vision Research Foundation

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+91-44-42271500


Dr M Bharathselvi, PhD

Senior Scientist / In-charge of HPLC and Mass-Spectrometry
Department of Biochemistry and Cell Biology



I started my research career in vision research foundation from the year 2005. During these years I attempted to understand the biochemistry behind retinal vascular biology. I worked on patients biological samples with retinal diseases like Eales disease, Age related macular degeneration and diabetic retinopathy. We have discovered mechanisms by which the non-protein amino acid homocysteine becomes a constituent of proteins. I have experience in handling cell lines and used them as models to study the biochemical mechanism of homocysteine and oxidative stress. I standardized methods to quantify enzymes, trace elements and biochemical molecules implicated in retinal vascular pathology by HPLC, atomic absorption spectroscopy. Recent advances in mass spectrometry (MS) based proteomics now enable the investigation of nearly complete proteomes and this technology has evolved into the method of choice for the identification of proteins as well as their site-specific posttranslational modifications techniques in the field of biomedical research, in order to understand the function, interaction and localization of each protein in the cell and its changes in disease states. Currently I am involved in high-throughput mass spectrometry proteomic method development.


Educational Qualifications:

I passed my under graduation in microbiology from the university of madras and pursued post-graduation in Medical laboratory technology from BITS, Pilani Rajasthan and then did my Ph.D degree in under the guidance of Prof.KN.Sulochana in 2016 from BITS, Pilani Rajasthan. My thesis work was on understanding the “Biochemical Mechanisms of Homocysteine and Its Role in Retinal Vascular diseases”.


Professional Training

  • Attended Seminar on Frontiers in MS technology and application spinco (2016)

  • Attended Seminar on recent trends in mass spectrometry and chromatography, spinco (2015)

  • Trained in mass spectrometer analysis xevo G2-S QTOF MS waters (2011)

  • Attended Seminar on LC/GC/ICP/MS conducted by Agilent technologies at Le Royal Merridian 2005

Teaching and training experience:

  1. Involved in taking theory and Practical classes for the BSc and MScMLT students , Conducting biochemistry practical classes for the Optometry students

  2. Hands on summer training: Involved in training the students on the instrumentation and its application

  3. Guiding short term project students in dissertation:
    1. Optimization of HPLC Analysis of ADMA, NMMA in Blood Samples

    2. Vitamin D estimation by HPLC in Ocular disease

    3. Dopamine and Serotonin estimation by HPLC in Ocular disease
  • Homocysteine and homocysteine thiolactone mediated dysfunction in retinal diseases.

  • Role in Oxidative stress in retinal vascular pathology

  • Trace element in the pathogenesis of retinal vascular diseases.

  • Mass spectrometry based proteomics.

High Pressure Liquid Chromatography work flow overview

Sample preparation


Ultra filtration

Solid-phase extraction


LC


Data Analysis



Analysis of


  • Aminoacids, peptides,
  • Steroids,
  • Vitamins,
  • Metabolites and Drugs

Project works carried out in HPLC
  1. Optimization of HPLC Analysis of N-Methyl arginine derivatives in Blood Samples
  2. Estimation of amino acids in Tear samples of Stevens Johnson syndrome.
  3. Estimation of Homocysteine Thiolactone in Eales’ Disease and Age related macular degeneration
  4. Estimation of Ornithine and Taurine in blood samples.
  5. Estimation of Histidine decarboxylase – Histidine, Histamine and Arginine in Hela Cell lines.
  6. Determination of collagen Cross- linker – Lysinonorleucine in cornea tissue of Keratoconus
  7. Neurotransmitters – Dopamine, Melatonin and Serotonin estimation by HPLC in myopia cases.
  8. Estimation of Lutein by HPLC in Ocular disease.
  9. Serum Vitamin D Levels by HPLC Analysis in Ocular Sarcoidosis and Uveitis,
  10. Serum Amino acids Levels in blood sample of Diabetic retinopathy .
  11. Serum and Tear Amino acids in Keratoconus disease.
  12. Determination of collagen Cross- linkers in Keratoconus.
  13. Determination of Elastin Cross-linkers – Desmosine and Isodesmosine in Pseudo-exfoliation Syndrome.
  14. Estimation of Dexamethasone in tissue samples such as sclera, conjunctiva and tenon of eye.
  15. Determination of Homocysteine and 7 amino acids in methionine pathway .

Research Highlights:

Homocysteine – binding with GCL


Proteins



Peptide Imaging




Google Scholar : Check out this link

  1. Manohar Rebecca, Krishnamoorthy Sripriya, M.Bharathselvi, B. Shantha, Lingam Vijaya, Narayanasamy Angayarkanni. Increased Desmosine in the lens capsules is associated with augmented elastin turnover in Pseudoexfoliation syndrome. Experimental Eye Research 215 (2022) 108898.

  2. Aberami,Nikhalashree, Bharathselvi M, Biswas J, Sulochana KN, KCoral,*Elemental concentrations in Choroid-RPE and retina of human eyes with age-related macular degeneration. Experimental Eye Research 186 (2019) 107718.

  3. SR Bharathi Devi, K Coral, M Bharathselvi, K Gayathree, S Sivasankar, J Biswas, P Rishi, S Natarajan, S. S.Badrinath, Angayarkanni. Case report on two diabetic donor eyes with no retinopathy: Clinicopathological and molecular studies. Indian Journal of Opthalmology. 2019.

  4. Bharathselvi M, Biswas S, Raman R, Selvi R, Coral K, Narayanansamy A, Ramakrishnan S, Sulochana KN.Authors' Response. Homocysteine & its metabolite homocysteine-thiolactone & deficiency of copper in patients with age related macular degeneration - A pilot study Indian J Med Res. 2017 Sep;146(3):431-432.

  5. Bharathselvi M, Biswas S, Raman R, Selvi R, Coral K, Narayanansamy A, Ramakrishnan S, Sulochana KN Homocysteine & its metabolite homocysteine-thiolactone & deficiency of copper in patients with age related macular degeneration - A pilot study. Indian J Med Res. 2016 Jun;143(6):756-762.

  6. Saravanan R, Bharathselvi M, Babu AC, Biswas J, Sulochana KN.The 88 kDa Eales' protein in serum is a complex of haptoglobin, complement C3 and galectin-1 as identified by liquid chromatography coupled mass spectrometry. Proteomics Clin Appl. 2016 Oct 14.

  7. Gomathy N, Bharathselvi M, Saravanan.R and KN.Sulochana.  Localization of CTR1 – copper transporters in Eales’ disease – Ocular immunology and inflammation. Journal of Ocular immunology and inflammation. 2016.

  8. Bharathselvi.M, V.Srinivasan, P.Rishi, Sulochana N. K. Increased Vitreous Heme Oxygenase Activity is Associated with Proliferative Diabetic Retinopathy. Ind J Clin Biochem. 2015.

  9. M.Bharathselvi,J.Biswas,R.Selvi,K.Coral,Angayarkanni.N,S.Ramakrishnan,andK.N.Sulochana.Increased Homocysteine, Homocysteine-Thiolactone, Protein Homocysteinylation, Oxidative Stress in circulation of patients with Eales’ Disease. Annals of clinical Biochemistry. Jun 2013.

  10. N.Gomathy; S.R Bharathidevi; M.Bharathselvi; V.Harish; KN.Sulochana. CTR1 silencing inhibits angiogenesis by limiting copper entry in endothelial cells.  PLOS ONE. 2013.

  11. R.Selvi, M.BharathSelvi, A.V.Saijyothi, J.Biswas, N.Angayarkanni and S. Ramakrishnan. Clinical and Biochemical benefits of administration of vitamins E & C in patients with Eale’s Disease. BIOMEDICINE. 2012.

  12. Coral K, Angayarkanni N, Gomathy N, Bharathselvi.M, Pukhraj R, Rupak R. Homocysteine levels in the vitreous of proliferative diabetic retinopathy and rhegmatogenous retinal detachment: its modulating role on lysyl oxidase. Invest Ophthalmol Vis Sci. 2009.

  13. Coral K, Angayarkanni N, Madhavan J, Bharathselvi.M, Ramakrishnan S, Nandi K, Rishi P, Kasinathan N, Krishnakumar S. Lysyl oxidase activity in the ocular tissues and the role of LOX in proliferative diabetic retinopathy and rhegmatogenous retinal detachment. Invest Ophthalmol Vis Sci. 2008.

  14. Bharathselvi.M, Selvi R, Sivaramakrishna R, KN.Sulochana. Biochemical Mechanisms of Homocysteine in Eye Disease. 2008.

  15. Selvi R, Angayarkanni N, Bharathselvi.M, Sivaramakrishna R, Anisha T,Jyotirmoy B, Vasanthi B. Increase in Fe3+/Fe2+ ratio and iron-induced oxidative stress in Eales disease and presence of ferrous iron in circulating transferrin.

2012
Travel grant in the ASIA- ARVO IERG Conference at Hyderabad


2013
Travel grant in the ASIA- ARVO IERG Conference at Hyderabad


Dr M Bharathselvi, PhD
Senior Scientist / In-charge of HPLC and Mass-Spectrometry
RS Mehta Jain Department of Biochemistry and Cell Biology
Vision Research Foundation,
41/18 College Road, Nungambakkam
Chennai – 600 006.
Ph: +91-(044) 28271616 Extn: 1858 / 1304
Email: drbharathselvi@snmail.org / bharathselvi363@gmail.com